{"product_id":"a-toolkit-to-study-nuclear-dilantha-perera-9783736978478","title":"A toolkit to study nuclear transport pathways: nanobody-mediated inhibition of importin \u0026#946;-related nuclear transport receptors (NTRs)","description":"Eukaryotic cells are divided into a nuclear and a cytoplasmic compartment. This separates transcription from translation and makes gene expression dependent on nucleocytoplasmic transport. The members of the importin β superfamily function as shuttling nuclear transport receptors (NTRs) that recognize and actively transport cargoes through nuclear pores. An estimated 5 000 to 10 000 different human proteins are subject to active nuclear transport. Numerous cargo\/NTR pairs have been identified, however, we are still far from a complete understanding as it has been very challenging to setup a systematic in vivo analysis that integrates the impact of all transport pathways. In this study, we obtained anti-NTR nanobodies against TRN1, Xpo4, Xpo7, and CAS. Our aim was to identify nanobodies, and prepare nanobody fusions, that impede nuclear pore-passage of the targeted NTR and thus, interrupt a given transport cycle. These nanobody fusions were observed to inhibit the partition of NTR\/cargo complexes into a reconstituted FG phase. We also observed that the nanobodies and nanobody fusions inhibit NTR transport in permeabilized cells.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Dilantha Perera\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 3736978472\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9783736978478\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Cuvillier\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 08\/07\/2023\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 136\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 0.38lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 8.27h x 5.83w x 0.29d","brand":"Dilantha Perera","offers":[{"title":"Paperback","offer_id":49173818704127,"sku":"9783736978478","price":51.13,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_088bb681-c709-4a12-88da-d97a67b7c950.jpg?v=1788956446","url":"https:\/\/www.whiterainbookhouse.com\/products\/a-toolkit-to-study-nuclear-dilantha-perera-9783736978478","provider":"WR Book House","version":"1.0","type":"link"}