{"product_id":"alterations-of-the-sister-chromatid-marlies-meisel-9783638910415","title":"Alterations of the Sister Chromatid Exchange frequency in peripheral lymphocytes caused by an Ironman triathlon","description":"Diploma Thesis from the year 2007 in the subject Medicine - Human Genetics, grade: 1,0, University of Vienna (Institut für Ernährungswissenschaften), language: English, abstract: The aim of the present study was to investigate the effect of a triathlon (3.8 km swim, 180 km cycle, 42,2 km run) on the genomic stability of nine highly trained non- professional athletes. Therefore, the SCE assay, a relevant biological response marker for genotoxicity in human biomonitoring studies [PENDZICH et al., 1997] was performed using peripheral lymphocytes, on account of their effortless accessibility [WILKOSCY and RYNARD, 1990]. Duplicate lymphocyte cell cultures, of each participant, were incubated for 72 h (37 C, 5% CO2) according to a short-term human lymphocyte cell culture. For each participant at least 50 metaphases, containing 43-46 chromosomes were scored, to evaluate the mean SCE frequency. The number of SCEs per cell was calculated to a chromosome set of a normal diploid human cell, containing 46 chromosomes. In the present study the alteration of SCE frequency, 48 h pre- and 24 h postrace was evaluated. As an additional endpoint Top 5 HFCs (highest five absolute SCE means) were assayed. It could be demonstrated that both the total mean SCE frequency and the mean Top 5 HFC frequency (n=9) 24 h postrace were significantly decreased (*p\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Marlies Meisel\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 3638910415\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9783638910415\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Grin Verlag\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 02\/20\/2008\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 92\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 0.29lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 8.27h x 5.83w x 0.22d","brand":"Marlies Meisel","offers":[{"title":"Paperback","offer_id":47244292325631,"sku":"9783638910415","price":62.9,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_48ad394f-e613-47bb-a162-0c11f79511e7.jpg?v=1758135389","url":"https:\/\/www.whiterainbookhouse.com\/products\/alterations-of-the-sister-chromatid-marlies-meisel-9783638910415","provider":"WR Book House","version":"1.0","type":"link"}