{"product_id":"ampk-dietbert-neumann-9781493975976","title":"Ampk: Methods and Protocols","description":"\u003cp\u003eThis volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cbr\u003eCutting edge and thorough, \u003ci\u003eAMPK: Methods and Protocols\u003c\/i\u003e is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Dietbert Neumann\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1493975978\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781493975976\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 02\/26\/2018\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 626\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 2.92lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 1.38d","brand":"Dietbert Neumann","offers":[{"title":"Hardcover","offer_id":48088400134399,"sku":"9781493975976","price":109.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_d71d9d87-7a5f-4671-a77d-95bd46ff7e77.jpg?v=1769104620","url":"https:\/\/www.whiterainbookhouse.com\/products\/ampk-dietbert-neumann-9781493975976","provider":"WR Book House","version":"1.0","type":"link"}