{"product_id":"an-ex-vivo-3d-tumor-lili-yang-9789994982899","title":"An Ex Vivo 3D Tumor Microenvironment-Mimicry Culture to Study TAM Modulation","description":"Tumor-associated macrophages (TAMs) accumulate in the solid tumor microenvironment (TME) and have been shown to promote tumor growth and dampen antitumor immune responses. TAM-mediated suppression of T-cell antitumor reactivity is considered to be a major obstacle for many immunotherapies, including immune checkpoint blockade and adoptive T\/CAR-T-cell therapies. An ex vivo culture system closely mimicking the TME can greatly facilitate the study of cancer immunotherapies. Here, we report the development of a 3D TME-mimicry culture that is comprised of the three major components of a human TME, including human tumor cells, TAMs, and tumor antigen-specific T cells. This TME-mimicry culture can readout the TAM-mediated suppression of T-cell antitumor reactivity, and therefore can be used to study TAM modulation of T-cell-based cancer immunotherapy. As a proof-of-principle, the studies of a PD-1\/PD-L1 blockade therapy and a MAO-A blockade therapy were performed and validated.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Lili Yang,Yan-Ruide Li\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 9994982893\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9789994982899\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Eliva Press\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 09\/22\/2022\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 70\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 0.31lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 9.00h x 6.00w x 0.18d","brand":"Lili Yang","offers":[{"title":"Paperback","offer_id":48113895014655,"sku":"9789994982899","price":45.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_d7ddd453-0a40-4626-8a11-e15896ec9941.jpg?v=1769617815","url":"https:\/\/www.whiterainbookhouse.com\/products\/an-ex-vivo-3d-tumor-lili-yang-9789994982899","provider":"WR Book House","version":"1.0","type":"link"}