{"product_id":"bacterial-protein-secretion-systems-laure-journet-9781493970315","title":"Bacterial Protein Secretion Systems: Methods and Protocols","description":"\u003cp\u003eThis volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and\/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003eAuthoritative and cutting-edge, \u003ci\u003eBacterial Protein Secretion Systems: Methods and Protocol \u003c\/i\u003eaims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.\u003cp\u003e\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Laure Journet\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1493970313\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781493970315\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 07\/01\/2017\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 528\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 2.56lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 1.19d","brand":"Laure Journet","offers":[{"title":"Hardcover","offer_id":47424404488447,"sku":"9781493970315","price":249.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_aeb1547d-4e47-4b5f-854a-c91063098eb3.jpg?v=1761530011","url":"https:\/\/www.whiterainbookhouse.com\/products\/bacterial-protein-secretion-systems-laure-journet-9781493970315","provider":"WR Book House","version":"1.0","type":"link"}