{"product_id":"bioassays-in-experimental-and-preclinical-karuppusamy-arunachalam-9781071612323","title":"Bioassays in Experimental and Preclinical Pharmacology","description":"\u003cp\u003e\u003cb\u003e1. \u003c\/b\u003e\u003cb\u003eOverview\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. \u003c\/b\u003e\u003cb\u003eCell Culture Assays\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Cell Cycle Assay: Propidium Iodide Marking\u003c\/p\u003e \u003cp\u003e2.2 Apoptosis Assay: Annexin V\/Propidium Iodide Marking\u003c\/p\u003e \u003cp\u003e2.3 Cytotoxicity in Caco-2 cells by the Alamar blue method\u003c\/p\u003e \u003cp\u003e2.4 Viability in Caco-2 cells stimulated with lipopolysaccharide (LPS)\u003c\/p\u003e \u003cp\u003e2.5 Determination of Nitric oxide (NO) by \u003ci\u003ein vitro \u003c\/i\u003eexperiment\u003c\/p\u003e \u003cp\u003e2.6 Analysis of specific cytotoxicity against RAW 264.7 for determination anti-inflammatory activity \u003ci\u003ein vitro\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.7 Scratching of Fibroblast cells (scratch assay)\u003c\/p\u003e \u003cp\u003e2.8 Evaluation of the in vitro inflammatory response in AGS cells\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. \u003c\/b\u003e\u003cb\u003ePhytochemical Analysis\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Preliminary phytochemical assays\u003c\/p\u003e \u003cp\u003e3.2 Quantitative analysis of secondary metabolites\u003c\/p\u003e \u003cp\u003e3.3 Thin Layer Chromatography Analysis\u003c\/p\u003e \u003cp\u003e3.4 Analysis by High Performance Liquid Chromatography (HPLC)\u003c\/p\u003e \u003cp\u003e3.5 Mass spectrometry\u003c\/p\u003e 3.6 Electrospray Ionization Mass Spectrometry (ESI-MS) analysis\u003cp\u003e\u003c\/p\u003e \u003cp\u003e3.7 Identification of phenolic compounds by HPLC-LC-ESI- MS\/MS\u003c\/p\u003e 3.8 Quantitative determination of total alkaloids content by HPLC\u003cp\u003e\u003c\/p\u003e \u003cp\u003e3.9 Quantitative determination of total alkaloids content by HPLC\u003c\/p\u003e \u003cp\u003e4. \u003cb\u003ePreclinical Drug Dose Calculation\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Conversion based on normalization of dose-to body surface area\u003c\/p\u003e 4.2 Determination of animal equivalent dose\u003cp\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. \u003c\/b\u003e\u003cb\u003eToxicology Studies (In Vitro and In Vivo)\u003c\/b\u003e\u003c\/p\u003e 5.1 Cytotoxicity evaluation using Alamar blue\u003cp\u003e\u003c\/p\u003e \u003cp\u003e5.2 Micronucleus test\u003c\/p\u003e 5.3 Comet test\u003cp\u003e\u003c\/p\u003e \u003cp\u003e5.4 Effect on cell cycle\u003c\/p\u003e \u003cp\u003e5.5 \u003ci\u003eIn vivo \u003c\/i\u003eacute toxicity study\u003c\/p\u003e \u003cp\u003e5.6 Subchronic toxicity (repeated doses)\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. \u003c\/b\u003e\u003cb\u003eStandard Procedure for Anesthesia in Preclinical Experiments\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Planning and anesthetic care\u003c\/p\u003e \u003cp\u003e6.2 Pre-Procedure\u003c\/p\u003e \u003cp\u003e6.3 Experimental Procedure\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. \u003c\/b\u003eGeneral Considerations and Collection of Animal Blood\u003c\/p\u003e \u003cp\u003e7.1 Vein blood collection\u003c\/p\u003e \u003cp\u003e7.2 Tail vein blood collection\u003c\/p\u003e \u003cp\u003e7.3 Cardiac puncture blood collection\u003c\/p\u003e \u003cp\u003e7.4 Posterior vena cava\u003c\/p\u003e 7.5 Orbital sinus\u003cp\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. \u003c\/b\u003e\u003cb\u003eAnimal Experiments on the Cardiovascular System\u003c\/b\u003e\u003c\/p\u003e 8.1 Obtaining electrocardiogram (ECG) and blood pressure (BP) signals\u003cp\u003e\u003c\/p\u003e \u003cp\u003e8.2 Myocardial ischemia induction\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. \u003c\/b\u003e\u003cb\u003eAnimal Experiments on Ulcerative Colitis\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Ulcerative colitis induced by 2,4,6-Trinitrobenzenesulfonic acid (TNBS)\u003c\/p\u003e 9.2 Chronic ulcerative colitis induced by Dextran Sodium Sulfate (SSD) 2%\u003cp\u003e\u003c\/p\u003e \u003cp\u003e9.3 Colitis with recurrence (Chronic)\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. \u003c\/b\u003e\u003cb\u003eExperiments on Anti-Bacterial Activities\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Antibacterial activity by \u003ci\u003ein vitro \u003c\/i\u003eassays\u003c\/p\u003e 10.2 Animal Experiments on antibacterial activity\u003cp\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. \u003c\/b\u003e\u003cb\u003eExperiments on Anti-Fungal Activities\u003c\/b\u003e\u003c\/p\u003e 11.1 Agar Disc Diffusion Method\u003cp\u003e\u003c\/p\u003e \u003cp\u003e11.2 Minimum Inhibitory Concentration (MIC)\u003c\/p\u003e 11.3 Sorbital test\u003cp\u003e\u003c\/p\u003e \u003cp\u003e11.4 \u003ci\u003eNeurospora crassa \u003c\/i\u003eassay\u003c\/p\u003e \u003cp\u003e11.5 Effect of Ergosterol on MIC\u003c\/p\u003e \u003cp\u003e11.6 Test of Β1,3-D-Glucan Synthase (GS)\u003c\/p\u003e \u003cp\u003e11.7\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Karuppusamy Arunachalam, Sreeja Puthanpura Sasidharan\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1071612328\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781071612323\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 02\/17\/2021\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 271\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.50lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.20h x 7.70w x 0.70d\u003c\/p\u003e","brand":"Karuppusamy Arunachalam","offers":[{"title":"Hardcover","offer_id":44127016288511,"sku":"9781071612323","price":179.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_2691183d-2d42-4833-8f58-390f486b5bc2.jpg?v=1687451404","url":"https:\/\/www.whiterainbookhouse.com\/products\/bioassays-in-experimental-and-preclinical-karuppusamy-arunachalam-9781071612323","provider":"WR Book House","version":"1.0","type":"link"}