{"product_id":"calcium-techniques-jan-b-parys-9781936113583","title":"Calcium Techniques: A Laboratory Manual","description":"Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses. To create spatiotemporally distinct Ca2+ signals, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved. \u003cp\u003e\u003c\/p\u003eThis laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice. \u003cp\u003e\u003c\/p\u003eSpecific chapters are devoted to Ca2+ signaling techniques in non-mammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Jan B. Parys\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1936113589\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781936113583\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Cold Spring Harbor Laboratory Press\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 10\/31\/2013\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 608\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 3.70lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.80h x 8.50w x 1.10d","brand":"Jan B. Parys","offers":[{"title":"Paperback","offer_id":44761326944511,"sku":"9781936113583","price":28.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_c7d25ada-5fac-4ccc-a3e7-bb67e4442a44.jpg?v=1706681013","url":"https:\/\/www.whiterainbookhouse.com\/products\/calcium-techniques-jan-b-parys-9781936113583","provider":"WR Book House","version":"1.0","type":"link"}