{"product_id":"cell-free-gene-expression-ashty-s-karim-9781071620007","title":"Cell-Free Gene Expression: Methods and Protocols","description":"This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cbr\u003eAuthoritative and practical, \u003ci\u003eCell‐Free Gene Expression: Methods and Protocols\u003c\/i\u003e serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Ashty S. Karim\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1071620002\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781071620007\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 01\/07\/2023\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 435\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.73lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.92d","brand":"Ashty S. Karim","offers":[{"title":"Paperback","offer_id":44120675680511,"sku":"9781071620007","price":169.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_bf7f4015-df7d-4ba9-b7e2-a54f8497d1df.jpg?v=1687415649","url":"https:\/\/www.whiterainbookhouse.com\/products\/cell-free-gene-expression-ashty-s-karim-9781071620007","provider":"WR Book House","version":"1.0","type":"link"}