{"product_id":"crispr-guide-rna-design-tudor-a-fulga-9781071606896","title":"Crispr Guide RNA Design: Methods and Protocols","description":"\u003cp\u003e\u003cb\u003ePart I: In Silico Design and Optimization of Guide RNA Sequences\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e1. Cloud-Based Design of Short Guide RNA (sgRNA) Libraries for CRISPR Experiments\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Florian Heigwer and Michael Boutros\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. Web-Based CRISPR Toolkits: Cas-OFFinder, Cas-Designer, and Cas-Analyzer\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Gue-Ho Hwang, Jin-Soo Kim, and Sangsu Bae\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cb\u003ePart II: Chemically-Modified Guide RNAs\u003c\/b\u003e\u003cp\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. Chemical Modification of Guide RNAs for Improved CRISPR Activity in CD34+ Human Hematopoietic Stem and Progenitor Cells\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Jenny Shapiro, Adi Tovin, Ortal Iancu, Daniel Allen, and Ayal Hendel\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e4. Gene Disruption Using Chemically-Modified CRISPR-Cpf1 RNA\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Moira A. McMahon and Meghdad Rahdar\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. 'Split-and-Click' sgRNA\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Lapatrada Taemaitree, Arun Shivalingam, Afaf H. El-Sagheer, and Tom Brown\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. Chimeric DNA-RNA Guide RNA Designs\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Shuhan Lu, Ying Zhang, and Hao Yin\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III: Expanding the CRISPR Toolbox \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. Harnessing tRNA for Processing Ability and Promoter Activity\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e David J.H.F. Knapp and Tudor A. Fulga\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. Targeting Noncoding RNA Domains to Genomic Loci with CRISPR-Display: Guidelines for Designing, Building, and Testing sgRNA-ncRNA Expression Constructs\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e David M. Shechner\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Quentin R.V. Ferry\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. \u003c\/b\u003e\u003cb\u003eGene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12a\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Ha Rim Shin, Jiyeon Kweon, and Yongsub Kim\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart IV: Characterization of CRISPR Efficacy and Specificity\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. \u003c\/b\u003e\u003cb\u003eMethods for Measuring CRISPR\/Cas9 DNA Cleavage in Cells\u003c\/b\u003e\u003c\/p\u003e Christopher R. Cromwell, Juan Jovel, and Basil P. Hubbard\u003cp\u003e\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e12. In Vitro Assays for Comparing the Specificity of First- and Next-Generation CRISPR\/Cas9 Systems\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Christopher R. Cromwell and Basil P. Hubbard\u003c\/p\u003e \u003cp\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13. Profiling Genome-Wide Specificity of CRISPR-Cas9 Using Digenome-Seq\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Daesik Kim and Jin-Soo Kim\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e14. Detection of CRISPR\/Cas9-Generated Off-Target Effect by Integration-Defective Lentiviral Vector\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Xiaoling Wang, Youjun Wu, and Jiing-Kuan Yee\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e15. Genome-Wide CRISPR Off-Target DNA Break Detection by the BLISS Method\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Roberto Ballarino, Britta A.M. Bouwman, and Nicola Crosetto\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Tudor A. Fulga\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1071606891\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781071606896\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 09\/16\/2021\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 286\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.15lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.62d","brand":"Tudor A. Fulga","offers":[{"title":"Paperback","offer_id":44860387393791,"sku":"9781071606896","price":139.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_a382905b-294d-440e-b359-1d8d4e5cf558.jpg?v=1709135963","url":"https:\/\/www.whiterainbookhouse.com\/products\/crispr-guide-rna-design-tudor-a-fulga-9781071606896","provider":"WR Book House","version":"1.0","type":"link"}