{"product_id":"data-driven-modeling-of-molecular-interactions-patrick-weber-9783832540234","title":"Data-Driven Modeling of Molecular Interactions at the Trans-Golgi Network of Mammalian Cells","description":"In this thesis simulation models have been developed and validated to investigate the protein-lipid interaction mechanisms at the trans-Golgi network (TGN) of mammalian cells. In two systems biological studies, ordinary differential equation models were used to examine the interactions between the lipids involved in the sphingomyelin synthase 1 reaction at the TGN and the proteins involved in the regulation of non-vesicular Endoplasmic reticulum to TGN ceramide transfer. These systems include the lipids ceramide, phosphatidyl choline, diacylglycerol and sphingomyelin and the proteins protein kinase D (PKD), phosphatidyl inositol-4-kinase III ss and ceramide transfer protein (CERT), respectively. The final results comprise a quantitative model of this network, and a comparison of competing hypotheses regarding the mechanism of ceramide transfer. Major biological findings are that PKD and CERT work together in a cooperative manner to perform ceramide transfer by forming a positive feedback regulation. Important methodological side results of this thesis are a novel absolute quantification scheme for proteins via Western Blot data and a Bayesian experiment design method.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Patrick Weber\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 3832540237\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9783832540234\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Logos Verlag Berlin\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 07\/20\/2015\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 125\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback","brand":"Patrick Weber","offers":[{"title":"Paperback","offer_id":48450133655807,"sku":"9783832540234","price":60.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_8f483f59-560d-46ad-b7ee-c3c781598e12.jpg?v=1777266357","url":"https:\/\/www.whiterainbookhouse.com\/products\/data-driven-modeling-of-molecular-interactions-patrick-weber-9783832540234","provider":"WR Book House","version":"1.0","type":"link"}