{"product_id":"dnazymes-gerhard-steger-9781071620465","title":"Dnazymes: Methods and Protocols","description":"Acknowledgement...Preface...Table of Contents...Contributing Authors...\u003cbr\u003ePart I SELEX1. A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzymeWenjing Li, Yanwei Cao, and Renjun Pei\u003cbr\u003e2. Selection of Aptamer for N-methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzymeLuyan Yang, Yanwei Cao, and Renjun Pei\u003cbr\u003e3. PCR Methods for the Generation of Catalytic ssDNADeni Szokoli, Kristian K. Le Vay, and Hannes Mutschler\u003cbr\u003ePart II Design - Kinetics\/Performance\/Turnover\u003cbr\u003e4. Design of a DNAzyme: Prediction of mRNA Regions Accessible to a DNAzymeGerhard Steger and Julian Victor\u003cbr\u003e5. Fluorescence-Based Kinetic Measurements for RNA-Cleaving DNAzymesHannah Rosenbach and Gerhard Steger\u003cbr\u003e6. Stability and Activity of the 10-23 DNAzyme under Molecular Crowding ConditionsNina Kirchg舖sler, Hannah Rosenbach, and Ingrid Span\u003cbr\u003e7. Quantifying the Number and Affinity of Mn2+-Binding Sites with EPR SpectroscopyChristine Wuebben and Olav Schiemann\u003cbr\u003ePart III Biophysical Characterization of DNAzymes\u003cbr\u003e8. Obtaining Crystals of Nucleic Acids in Complex with the Protein U1A Using the Soaking MethodHannah Rosenbach and Ingrid Span\u003cbr\u003e9. Crystallization and Structural Determination of 8-17 DNAzymeHehua Liu, Song Mao, Jia Sheng, and Jianhua Gan\u003cbr\u003e10. Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and FunctionJan Borggr臟e and Manuel Etzkorn\u003cbr\u003e11. Molecular Modeling and Simulations of DNA and RNA: DNAzyme as a Model SystemChristoph G.W. Gertzen and Holger Gohlke\u003cbr\u003ePart IV Further Biophysical Methods\u003cbr\u003e12. Single-Molecule Kinetic Studies of Nucleic Acids by Fster Resonance Energy TransferM駘odie C.A.S. Hadzic, Roland K.O. Sigel, and Richard Bner\u003cbr\u003e13. Chemical Dual End-Labeling of Large RibozymesEsra Ahunbay, Fabio D. Steffen, Susann Zelger-Paulus, and Roland K. O. Sigel\u003cbr\u003e14. Spin Labeling of Long RNAs via Click Reaction and Enzymatic LigationMaria Francesca Vicino, Christine Wuebben, Mark Kerzhner, Michael Famulok, and Olav Schiemann\u003cbr\u003e15. Preparation of Site-Specifically Spin-Labeled RNA by In Vitro Transcription using an Expanded Genetic AlphabetLisa Bornewasser and Stephanie Kath-Schorr\u003cbr\u003e16. PELDOR Measurements on Nitroxide-Labelled OligonucleotidesTobias Hett and Olav Schiemann\u003cbr\u003ePart V Use of DNAzymes\u003cbr\u003e17. Sensing Metal Ions with Phosphorothioate-Modified DNAzymesPo-Jung Jimmy Huang and Juewen Liu\u003cbr\u003e18. Enhancement of Activity for Peroxidase-Mimicking DNAzyme by Covalent Attachment of Hemin to G-quadruplex-Forming Oligonucleotide Using Click ChemistryJoanna Kosman\u003cbr\u003e19. Generation of RNA with 2', 3'-cyclic Phosphates by Deoxyribozyme Cleavage in Frozen SolutionsKristian K. Le Vay and Hannes Mutschler\u003cbr\u003eSubject Index List...\u003cbr\u003e\u003col\u003e \u003c\/ol\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Gerhard Steger\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1071620460\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781071620465\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 03\/01\/2022\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 312\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.55lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.10w x 0.88d","brand":"Gerhard Steger","offers":[{"title":"Hardcover","offer_id":44066909716735,"sku":"9781071620465","price":219.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_1c35850d-8675-4174-b0b3-56b93c3f4de9.jpg?v=1685410058","url":"https:\/\/www.whiterainbookhouse.com\/products\/dnazymes-gerhard-steger-9781071620465","provider":"WR Book House","version":"1.0","type":"link"}