{"product_id":"ecoli-amit-setia-9783639026276","title":"E.coli: Probiotic for Piglet Diarrhoea","description":"Aim of this study was to select environmental E. coli isolates that pro-duced colicins against the swine pathogen E. coli K88+. In initial evaluation using a modified plate method with 18 colicinogenic E. coli constructs, colicins E3, E4, E5, E9, Ia, K and N were found to possess inhibitory activity against 12 ETEC K88+ strains. A total of 463 environmental isolates from cattle rumen, cattle feces, pig feces and hog manure-amended soil were screened for colicin production by a modified plate test. Further, colicinogenic isolates were screened for five toxin genes LT, STa, STb, VT1 and VT2 as well as K88 (F4) fimbriae using PCR reactions. Fourteen non-pathogenic isolates were subjected to characterization of colicin genes by PCR using 9 new primer sequences, antibiotic susceptibilities and substrate utilization. Two potential probiotic strains of E. coli, UM-2 and UM-7 which produced colicins that could utilize potato starch and inulin were selected for in-vitro competition with E. coli K88+ strain 2-12. In vitro competition between the synbiotics and E. coli K88+ revealed inhibition of E. coli K88+. Animal testing of these strains is currently being undertaken.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Amit Setia,Denis O. Krause\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 3639026276\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9783639026276\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e VDM Verlag Dr. Mueller E.K.\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 05\/27\/2008\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 116\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 0.36lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 9.00h x 6.00w x 0.24d","brand":"Amit Setia","offers":[{"title":"Paperback","offer_id":48113836523775,"sku":"9783639026276","price":52.92,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_04c7d3a1-9e7d-4c23-b258-c7f36d2b39fb.jpg?v=1769617501","url":"https:\/\/www.whiterainbookhouse.com\/products\/ecoli-amit-setia-9783639026276","provider":"WR Book House","version":"1.0","type":"link"}