{"product_id":"essential-genes-and-genomes-ren-zhang-9781071617229","title":"Essential Genes and Genomes: Methods and Protocols","description":"\u003cp\u003e\u003cb\u003e1. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Barbara Mair, Michael Aregger, Amy H.Y. Tong, Katherine S.K. Chan, and Jason Moffat\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. CRISPR\/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Kimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, and Jason R. Cantor\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. \u003c\/b\u003e\u003cb\u003eTarget Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Bert Kwanten, Jasper Neggers, and Dirk Daelemans\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e4. Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen \u003ci\u003eCandida albicans\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Lauren Wensing and Rebecca S. Shapiro\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. Identification of Essential Genes Using Sequential CRISPR and siRNA Screens\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Luke DeHart, Oliver P. Yockey, and Jesse Bakke\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Kohei Nishimura and Tatsuo Fukagawa\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Xihao Liao, Xin-Hui Xing, and Chong Zhang\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Ju-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, and Cheng-Cai Zhang\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. A Method of Transposon Insertion Sequencing in Comprehensively Identifying \u003ci\u003eVibrio vulnificus\u003c\/i\u003e Genes Required for Growth in Human Serum\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Miguel Carda-Di馮uez and Carmen Amaro\u003c\/p\u003e \u003cp\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of \u003ci\u003ePseudomonas aeruginosa\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Bradley E. Poulsen, Anne E. Clatworthy, and Deborah T. Hung\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. Analysis of \u003ci\u003eEscherichia coli\u003c\/i\u003e K1 Virulence Genes by Transposon-Directed Sequencing\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Alex J. McCarthy and Peter W. Taylor\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e12. Using Genome Scale Mutant Libraries to Identify Essential Genes\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Kevin S. Myers, Piyush Lal, Daniel R. Noguera, and Timothy J. Donohue\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e13. Identification and Analysis of Essential Genes in \u003ci\u003eStreptococcus mutans\u003c\/i\u003e with Transposon Sequencing\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Alejandro R. Walker and Robert C. Shields\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e14. The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle Systems\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Gayatri Sharma and Patrick D. Curtis\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e15. A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Ren Zhang\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1071617222\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781071617229\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 10\/29\/2022\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 434\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.70lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.91d\u003c\/p\u003e","brand":"Ren Zhang","offers":[{"title":"Paperback","offer_id":44071102644479,"sku":"9781071617229","price":169.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_fa5a2e9c-2830-4ebc-8b35-5172c4d4529b.jpg?v=1685434165","url":"https:\/\/www.whiterainbookhouse.com\/products\/essential-genes-and-genomes-ren-zhang-9781071617229","provider":"WR Book House","version":"1.0","type":"link"}