{"product_id":"experiments-in-bacterial-genetics-bossicamilligrundl-9781621824497","title":"Experiments in Bacterial Genetics: A Laboratory Manual","description":"Bacteria are powerful systems for genetic research. They are fast growing and easy to propagate and maintain, and many tools have been developed for their genomic analyses and genetic manipulation. The study of bacteria and their viruses allowed deciphering the genetic code and has shaped our understanding of fundamental biological processes such as DNA replication, transcription, and translation. \u003cp\u003e\u003c\/p\u003eThis manual provides a collection of experimental procedures for genetic research in bacteria. It describes basic methods for the isolation and propagation of bacteria and phages, purification of genomic DNA and plasmids, and introduction of DNA into bacteria. Diverse strategies for generating and confirming mutations and for creating gene deletions and gene fusions are described, using allelic exchange, random transposition, recombineering, and CRISPR-Cas technologies. Protocols for bacterial genome sequencing and resequencing, as well as for high-throughput mutant screening via transposon sequencing, are provided. \u003cp\u003e\u003c\/p\u003eAlthough specific bacterial species such as \u003cem\u003eEscherichia coli\u003c\/em\u003e, \u003cem\u003eSalmonella enterica\u003c\/em\u003e, \u003cem\u003eVibrio cholerae\u003c\/em\u003e, and \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e are used for some of the protocols, the methodologies herein described can readily be adapted for use with other bacteria. This manual thus represents a valuable resource for those seeking to perform genetic experiments in bacteria.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Bossi\/Camilli\/Grundl\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1621824497\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781621824497\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Cold Spring Harbor Laboratory Press\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 12\/31\/2023\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 352\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.97lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.92h x 8.54w x 0.78d","brand":"Bossi\/Camilli\/Grundl","offers":[{"title":"Paperback","offer_id":44540461580543,"sku":"9781621824497","price":95.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_957f23d1-4688-4c1c-8ce3-2148285341ae.jpg?v=1700588033","url":"https:\/\/www.whiterainbookhouse.com\/products\/experiments-in-bacterial-genetics-bossicamilligrundl-9781621824497","provider":"WR Book House","version":"1.0","type":"link"}