{"product_id":"fission-yeast-iain-hagan-9781621820826","title":"Fission Yeast: A Laboratory Manual","description":"Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. \u003cp\u003e\u003c\/p\u003eThis laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. \u003cp\u003e\u003c\/p\u003eThe well-studied fission yeast \u003cem\u003eSchizosaccharomyces pombe \u003c\/em\u003eis the focus throughout, but the emerging model \u003cem\u003eS. japonicus\u003c\/em\u003e-a larger, dimorphic species with several desirable characteristics-is also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Iain Hagan\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1621820823\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781621820826\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Cold Spring Harbor Laboratory Press\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 08\/17\/2016\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 490\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 2.90lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.90h x 8.50w x 1.10d","brand":"Iain Hagan","offers":[{"title":"Paperback","offer_id":48065758658815,"sku":"9781621820826","price":90.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_b97a1d30-746a-4a58-8369-be69ad8b22f2.jpg?v=1768677077","url":"https:\/\/www.whiterainbookhouse.com\/products\/fission-yeast-iain-hagan-9781621820826","provider":"WR Book House","version":"1.0","type":"link"}