{"product_id":"herpes-simplex-virus-russell-j-diefenbach-9781493998135","title":"Herpes Simplex Virus: Methods and Protocols","description":"This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR\/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e Cutting-edge and comprehensive, \u003ci\u003eHerpes Simplex Virus: Methods and Protocols, Second Edition\u003c\/i\u003e is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and\/or cellular-based approaches to study HSV. \u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Russell J. Diefenbach\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1493998137\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781493998135\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 10\/16\/2019\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 457\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 2.15lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.08h x 6.93w x 1.10d","brand":"Russell J. Diefenbach","offers":[{"title":"Hardcover","offer_id":48088404984063,"sku":"9781493998135","price":169.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_39287768-3488-4f54-9dbe-c264161db979.jpg?v=1769104648","url":"https:\/\/www.whiterainbookhouse.com\/products\/herpes-simplex-virus-russell-j-diefenbach-9781493998135","provider":"WR Book House","version":"1.0","type":"link"}