{"product_id":"heterologous-expression-of-membrane-proteins-isabelle-mus-veteau-9781071623701","title":"Heterologous Expression of Membrane Proteins: Methods and Protocols","description":"This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using \u003ci\u003eE. coli\u003c\/i\u003e as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cbr\u003eAuthoritative and up-to-date, \u003ci\u003eHeterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition\u003c\/i\u003e serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Isabelle Mus-Veteau\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1071623702\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781071623701\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 07\/02\/2023\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 467\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.85lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.98d","brand":"Isabelle Mus-Veteau","offers":[{"title":"Paperback","offer_id":44212935196927,"sku":"9781071623701","price":169.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_3ef49c00-ffe0-48da-9da4-79aa4ce17811.jpg?v=1692111123","url":"https:\/\/www.whiterainbookhouse.com\/products\/heterologous-expression-of-membrane-proteins-isabelle-mus-veteau-9781071623701","provider":"WR Book House","version":"1.0","type":"link"}