{"product_id":"in-vitro-guide-kevin-thomsone-9798268275445","title":"In Vitro Guide: Tissue Culture Protocols for Aquarium Plants: 50 Tissue Culture Protocols for Aquatic Plants","description":"\u003cp\u003e\u003cb\u003eIn Vitro Guide: Tissue Culture Protocols for Aquarium Plants - 50 Aquatic Plant Protocols for Successful Propagation\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUnlock the science of in vitro plant culture and take your aquascaping or aquatic plant business to the next level.\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eThe \u003cb\u003enew, updated edition\u003c\/b\u003e of \u003ci\u003eIn Vitro Guide: Tissue Culture Protocols for Aquarium Plants\u003c\/i\u003e is the ultimate hands-on reference for anyone serious about growing aquatic plants through sterile, laboratory-grade techniques. Designed for \u003cb\u003ehobbyists, commercial growers, and aquascaping professionals\u003c\/b\u003e, this comprehensive guide delivers \u003cb\u003e50 complete, step-by-step protocols\u003c\/b\u003e-each one \u003ci\u003etested, refined, and updated\u003c\/i\u003e for real-world success.\u003c\/p\u003e\u003cp\u003eEvery protocol now includes \u003cb\u003eexplicit sugar source formulations\u003c\/b\u003e (no longer assumed or omitted), ensuring precise and repeatable results across every media recipe. Whether you're using sucrose, glucose, or other carbohydrate sources, this edition specifies the exact concentration and context for each plant species-removing the guesswork and improving growth consistency.\u003c\/p\u003e\u003cbr\u003e\u003cb\u003eWhat You'll Learn\u003c\/b\u003e\u003cp\u003eInside, you'll find: \u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eDetailed explant preparation\u003c\/b\u003e and sterilization methods\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eMedia formulations\u003c\/b\u003e with exact macro\/micronutrient and sugar source details\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eOptimized hormone concentrations\u003c\/b\u003e for shoot induction, rooting, and multiplication\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eClear timing cycles\u003c\/b\u003e for subculture intervals and callus management\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eAcclimatization techniques\u003c\/b\u003e for converting from agar to submersed aquatic growth\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eLab setup advice\u003c\/b\u003e for scaling from DIY jars to professional in vitro production\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eEach protocol is built around \u003cb\u003epractical, tested lab workflows\u003c\/b\u003e adaptable to any scale-whether you're working from a home setup or a commercial facility.\u003c\/p\u003e\u003cbr\u003e\u003cb\u003ePlants Featured\u003c\/b\u003e\u003cp\u003eThis edition includes 50 of the most sought-after aquatic species, with verified formulations for: \u003cbr\u003e\u003cb\u003eRotala, Ludwigia, Bacopa, Limnophila, Hygrophila, Micranthemum, Hemianthus, Glossostigma, Hydrocotyle, Lilaeopsis, Marsilea, Eleocharis, Sagittaria, Helanthium, Juncus, Cryptocoryne, Echinodorus, Barclaya, Staurogyne, Pogostemon, Bolbitis, Anubias, Bucephalandra, and Taxiphyllum.\u003c\/b\u003e\u003c\/p\u003e\u003cbr\u003e\u003cb\u003eWhy This Guide Stands Out\u003c\/b\u003e\u003cp\u003eUnlike general plant tissue culture manuals, this book focuses entirely on \u003cb\u003eaquatic plants\u003c\/b\u003e, providing: \u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eSpecies-specific sugar sources\u003c\/b\u003e and hormonal ratios\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eConsistency-driven formulations\u003c\/b\u003e for higher multiplication and survival rates\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eWorkflows for contamination prevention\u003c\/b\u003e and sterile handling\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eDirect acclimation guidance\u003c\/b\u003e for aquarium-ready growth\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eScalable methods\u003c\/b\u003e for both personal and commercial propagation\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003cbr\u003e\u003cb\u003eWho It's For\u003c\/b\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eHobbyists\u003c\/b\u003e who want to eliminate pests, algae, and diseases while learning clean propagation.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eAquascapers\u003c\/b\u003e looking to self-produce rare or expensive plants.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cb\u003eCommercial growers\u003c\/b\u003e aiming for uniform, high-yield batches and professional-grade results.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e \u003cp\u003eIf you're ready to \u003cb\u003etransform your understanding of aquatic plants\u003c\/b\u003e and gain full control over their propagation-from sterile agar to lush aquarium growth-this updated edition is your \u003cb\u003eessential lab companion\u003c\/b\u003e.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eNow featuring verified sugar source data for every protocol-because precision starts with the right recipe.\u003c\/b\u003e\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Kevin Thomsone\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9798268275445\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Independently Published\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 10\/03\/2025\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 288\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 0.85lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 9.00h x 6.00w x 0.60d","brand":"Kevin Thomsone","offers":[{"title":"Paperback","offer_id":48089444122879,"sku":"9798268275445","price":29.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_6a243744-5974-495e-84ed-6c45e403e226.jpg?v=1769110292","url":"https:\/\/www.whiterainbookhouse.com\/products\/in-vitro-guide-kevin-thomsone-9798268275445","provider":"WR Book House","version":"1.0","type":"link"}