{"product_id":"membrane-trafficking-jingshi-shen-9781071622087","title":"Membrane Trafficking: Methods and Protocols","description":"\u003cp\u003e\u003cb\u003ePart I: The Exocytic Pathway\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1. Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Marine D. Camus and Stephane M. Camus\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Julia Dancourt and Gr馮ory Lavieu\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH)\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Cedric S. Asensio\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e4. Spatial and Temporal Control of Protein Secretion with Light\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Ashley M. Bourke and Matthew J. Kennedy\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Syed Mukhtar Ahmed\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Kunrong Mei, Di-Ao Liu, and Wei Guo\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Fei Kang and Herbert Y. Gaisano\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Jihong Gong, Xiaofei Yang, and Cong Ma\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. Live-Cell Super-Resolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Tong Wang and Fr馘駻ic A. Meunier\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Tamara Basta, Garry P. Morgan, Eileen T. O'Toole, Nalini R. Rao, Jeffrey N. Savas, and Michael H.B. Stowell\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Yinghui Liu, Ruyue He, Min Zhu, and Haijia Yu\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e12. Imaging Single Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)\u003c\/b\u003e\u003c\/p\u003e Yingke Xu, Luhong Jin, and Derek Toomre\u003cp\u003e\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II: The Endocytic Pathway\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13. Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Bijeta Prasai and Justin W. Taraska\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e14. Endocytosis Assays Using Cleavable Fluorescent Dyes\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Shifeng Wang, Chun Wan, Galen T. Squiers, and Jingshi Shen\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e15. Reconstituting and Purifying Assembly Intermediates of Clathrin Adaptors AP1 and AP2\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Bing Wang, Rui Yang, Yuan Tian, and Qian Yin\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e16. Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e Amulya Priya and Sunand\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Jingshi Shen\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1071622080\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781071622087\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 07\/13\/2022\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 407\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 2.09lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.94d\u003c\/p\u003e","brand":"Jingshi Shen","offers":[{"title":"Hardcover","offer_id":44129308115199,"sku":"9781071622087","price":249.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_25a975b7-daa7-4ce7-82b0-f7f6138369f8.jpg?v=1687463174","url":"https:\/\/www.whiterainbookhouse.com\/products\/membrane-trafficking-jingshi-shen-9781071622087","provider":"WR Book House","version":"1.0","type":"link"}