{"product_id":"metabolic-and-bioprocess-engineering-of-betina-da-silva-ribeiro-9783832531232","title":"Metabolic and Bioprocess Engineering of Production Cell Lines for Recombinant Protein Production","description":"Dissolved carbon dioxide has been identified as an important process parameter affecting cell growth, productivity and product quality (e.g. glycosylation) of recombinant proteins when exceeding critical levels, occurring especially in industrial large-scale cell culture processes due to the increased hydrostatic pressure. As CO\u003csub\u003e2\u003c\/sub\u003e can easily pass the cellular membrane and thereby influence intracellular pH, important cellular processes (e.g. cell cycle regulation, enzymes of TCA cycle) are directly influenced by pCO\u003csub\u003e2\u003c\/sub\u003e and dependent bicarbonate concentration. Consequently, process control strategies attend to keep pCO\u003csub\u003e2\u003c\/sub\u003e within physiological range. In a metabolic engineering approach an antibody producing CHO cell line stably expressing human carbonic anhydrase (hCAII), the enzyme that catalyzes the equilibrium of CO\u003csub\u003e2\u003c\/sub\u003e in aqueous solutions, was generated and used to characterize CO\u003csub\u003e2\u003c\/sub\u003e effects in simulated CO\u003csub\u003e2\u003c\/sub\u003e acid load and high CO\u003csub\u003e2\u003c\/sub\u003e levels as they occur in large scale mammalian cell culture. The cell line expressing active hCAII showed more rapid initial re-alkalinization of cytoplasm after induced CO\u003csub\u003e2\u003c\/sub\u003e acid load.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Betina Da Silva Ribeiro\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 3832531238\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9783832531232\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Logos Verlag Berlin\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 04\/16\/2012\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 160\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback","brand":"Betina Da Silva Ribeiro","offers":[{"title":"Paperback","offer_id":48450126708991,"sku":"9783832531232","price":81.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_892ddbce-49bc-49d2-b207-b32b63bf2dd2.jpg?v=1777266326","url":"https:\/\/www.whiterainbookhouse.com\/products\/metabolic-and-bioprocess-engineering-of-betina-da-silva-ribeiro-9783832531232","provider":"WR Book House","version":"1.0","type":"link"}