{"product_id":"molecular-methods-for-evolutionary-genetics-virginie-orgogozo-9781617792274","title":"Molecular Methods for Evolutionary Genetics","description":"\u003cp\u003ePart I. Characterizing the Genome\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e1. Genome Size Determination using Flow Cytometry of Propidium Iodide-stained Nuclei \u003c\/p\u003e\u003cp\u003e Emily E. Hare and J. Spencer Johnston\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e2. Chromosome Analysis in Invertebrates and Vertebrates \u003c\/p\u003e\u003cp\u003e David M. Rowell, Shu Ly Lim and Frank Grutzner\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e3. Genomic Libraries. I. Construction and Screening of Fosmid Genomic Libraries \u003c\/p\u003e\u003cp\u003e Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, and Simon W. Baxter\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e4. Genomic Libraries. II. Subcloning, Sequencing, and Assembling Large-insert Genomic DNA Clones \u003c\/p\u003e\u003cp\u003e Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, and Simon W. Baxter\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart II. Targeting Regions of the Genome\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e5. Reduced Representation Methods for Sub-genomic Enrichment and Next-generation Sequencing \u003c\/p\u003e\u003cp\u003e Jeffrey M. Good\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e6. Accessing the Transcriptome: How to Normalize mRNA Pools \u003c\/p\u003e\u003cp\u003e Heiko Vogel and Christopher W. Wheat\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e7. Transcriptome Sequencing Goals, Assembly, and Assessment \u003c\/p\u003e\u003cp\u003e Christopher W. Wheat and Heiko Vogel\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e8. Rapid Retrieval of DNA Target Sequences by Primer Extension Capture \u003c\/p\u003e\u003cp\u003e Adrian W. Briggs\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart III. Measuring Genetic Diversity\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e9. SNP Discovery and Genotyping for Evolutionary Genetics using RAD Sequencing \u003c\/p\u003e\u003cp\u003e Paul D. Etter, Susan Bassham, Paul A. Hohenlohe, Eric Johnson, and William A. Cresko\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e10. DNA Microarray-based Mutation Discovery and Genotyping \u003c\/p\u003e\u003cp\u003e David Gresham\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e11. Genotyping with Sequenom \u003c\/p\u003e\u003cp\u003e Martina Bradic, João Costa, and Ivo M. Chelo\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e12. Isolating Microsatellite Loci: Looking Back, Looking Ahead \u003c\/p\u003e\u003cp\u003e José A. Andrés and Steven M. Bogdanowicz\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e13. Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids using Array Oligo Selector \u003c\/p\u003e\u003cp\u003e Amy A. Caudy\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart IV. Obtaining Candidate Gene Sequences\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e14. Identification of Homologous Gene Sequences by PCR with Degenerate Primers \u003c\/p\u003e\u003cp\u003e Michael Lang and Virginie Orgogozo\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e15. Characterizing cDNA Ends by Circular RACE \u003c\/p\u003e\u003cp\u003e Patrick T. McGrath\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e16. Identification of DNA Sequences that Flank a Known Region by Inverse PCR \u003c\/p\u003e\u003cp\u003e Anastasios Pavlopoulos\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart V. Analyzing Candidate Gene Transcripts\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e17. Quantification of Transcript Levels with Quantitative RT-PCR \u003c\/p\u003e\u003cp\u003e Karen L. Carleton\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e18. Using Pyrosequencing to Measure Allele-specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences \u003c\/p\u003e\u003cp\u003e Patricia J. Wittkopp\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e19. Whole Mount In situ Hybridization of Sectioned Tissues of Species Hybrids to Detect Cis-Regulatory Changes in Gene Expression Pattern \u003c\/p\u003e\u003cp\u003e Ryo Futahashi\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e20. Identifying Fluorescently Labeled Single Molecules in Image Stacks using Machine Learning \u003c\/p\u003e\u003cp\u003e Scott Rifkin\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart VI. Testing Candidate Genes and Candidate Mutations\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e21. Experimental Approaches to Evaluate the Contributions of Candidate Cis-regulatory Mutations to Phenotypic Evolution\u003c\/p\u003e\u003cp\u003e Mark Rebeiz and Thomas M. Williams \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e22. Experimental Approaches to Evaluate the Contributions of Candidate Protein-coding Mutations to Phenotypic Evolution\u003c\/p\u003e\u003cp\u003e Jay F. Storz and Anthony J. Zera \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e23. Making Reporter Gene Constructs to Analyze Cis-regulatory Elements \u003c\/p\u003e\u003cp\u003e José Bessa and José Luis Gómez-Skarmeta\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e24. PCR-directed In vivo Plasmid Construction using Homologous Recombination in Baker's Yeast \u003c\/p\u003e\u003cp\u003e Erik C. Andersen\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e25. Pr\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Virginie Orgogozo\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1617792276\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781617792274\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 09\/29\/2011\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 503\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 2.30lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.10h x 7.00w x 1.30d\u003c\/p\u003e","brand":"Virginie Orgogozo","offers":[{"title":"Hardcover","offer_id":44125053649151,"sku":"9781617792274","price":169.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_dea97a55-94ba-4b5f-be2b-bffa2263cb86.jpg?v=1687440366","url":"https:\/\/www.whiterainbookhouse.com\/products\/molecular-methods-for-evolutionary-genetics-virginie-orgogozo-9781617792274","provider":"WR Book House","version":"1.0","type":"link"}