{"product_id":"monoclonal-antibodies-vincent-ossipow-9781627039918","title":"Monoclonal Antibodies: Methods and Protocols","description":"\u003cp\u003e\u003ci\u003eMonoclonal Antibodies: Methods and Protocols\u003c\/i\u003e, \u003ci\u003eSecond Edition\u003c\/i\u003e expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003eAuthoritative and practical, \u003ci\u003eMonoclonal Antibodies: Methods and Protocols, Second Edition\u003c\/i\u003e provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Vincent Ossipow\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1627039910\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781627039918\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 02\/12\/2014\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 575\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 2.72lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 1.25d","brand":"Vincent Ossipow","offers":[{"title":"Hardcover","offer_id":48519018643711,"sku":"9781627039918","price":329.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_ff0381f5-9376-416d-9b9a-5f8e9f2970d4.jpg?v=1778736520","url":"https:\/\/www.whiterainbookhouse.com\/products\/monoclonal-antibodies-vincent-ossipow-9781627039918","provider":"WR Book House","version":"1.0","type":"link"}