{"product_id":"protein-expression-in-mammalian-cells-james-l-hartley-9781493961603","title":"Protein Expression in Mammalian Cells: Methods and Protocols","description":"\u003cp\u003e\u003cb\u003e1. Why Proteins in Mammalian Cells?\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e James L. Hartley\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e2. \u003c\/b\u003e\u003cb\u003eLarge Scale Transfection of Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Lucia Baldi, David L. Hacker, Carine Meerschman, and Florian M. Wurm\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e3. \u003c\/b\u003e\u003cb\u003eSelection of High Expressing Mammalian Cells by Surface Display of Reporters\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Christine T. DeMaria\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e4. Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e5. Transfection of Difficult-to-Transfect Primary Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Oliver Gresch and Ludger Altrogge\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e6. \u003c\/b\u003e\u003cb\u003eStable Protein Expression in Mammalian Cells Using Baculoviruses\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vmarty, Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e7. \u003c\/b\u003e\u003cb\u003eUsing Matrix Attachment Regions to Improve Recombinant Protein Production\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, Pierre-Alain Girod, and Nicolas Mermod\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e8. \u003c\/b\u003e\u003cb\u003eControlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, and Tina M. Sauerwald\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e9. \u003c\/b\u003e\u003cb\u003ePost-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e10. Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, Rebecca Hamer, and Raymond J. Owens\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e11. \u003c\/b\u003e\u003cb\u003eGeneration of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Say Kong Ng\u003c\/p\u003e\u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e12. \u003c\/b\u003e\u003cb\u003eTools for Co-Producing Multiple Proteins in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e13. Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Paula Magnelli, Alicia Bielik, and Ellen Guthrie\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e14. \u003c\/b\u003e\u003cb\u003eStrategies for the Efficient Transfection of CHO-Cells with Plasmid DNA\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Renate Kunert and Karola Vorauer-Uhl\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e15. \u003c\/b\u003e\u003cb\u003eMethods for Constructing Clones for Protein Expression in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Takefumi Sone and Fumio Imamoto\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003e16. Optimizing Transient Recombinant Protein Expression in Mammalian Cells\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e James L. Hartley\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1493961608\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781493961603\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 08\/23\/2016\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 276\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.12lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.61d","brand":"James L. Hartley","offers":[{"title":"Paperback","offer_id":44124084732159,"sku":"9781493961603","price":119.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_da94ed58-52fd-4050-b89b-117a523ccf14.jpg?v=1687434652","url":"https:\/\/www.whiterainbookhouse.com\/products\/protein-expression-in-mammalian-cells-james-l-hartley-9781493961603","provider":"WR Book House","version":"1.0","type":"link"}