{"product_id":"riboflavin-production-with-ashbya-gossypii-susanne-katharina-schwechheimer-9783736998810","title":"Riboflavin production with Ashbya gossypii (Band 6)","description":"The fungus Ashbya gossypii is an important industrial producer of riboflavin, i.e. vitamin B2. Here, we developed and then used a highly sophisticated set-up of parallel 13C tracer studies with labeling analysis by GC\/MS, LC\/MS, 1D, and 2D NMR to resolve carbon fluxes and obtain a detailed picture of the underlying metabolism in the overproducing strain A. gossypii B2 during growth and riboflavin production from a complex industrial medium using vegetable oil as carbon source. Glycine was exclusively used as carbon-two - but not carbon-one (C1) - donor of the vitamin's pyrimidine ring due to the proven absence of a functional glycine cleavage system. Yeast extract was the main carbon source during growth, while still contributing 8 % overall carbon to riboflavin. Overall carbon flux from rapeseed oil into riboflavin equaled 80 %. Transmembrane formate flux simulations revealed that the C1-supply displayed a severe bottleneck during the initial riboflavin production, which was overcome in later phases of the cultivation by intrinsic formate accumulation. The transiently limiting C1-pool was successfully replenished by time-resolved feeding of formate or serine. This increased the intracellular precursor availability and resulted in a riboflavin titer increase of 45 %. This study is the first that successfully sheds light on carbon fluxes during the growth and riboflavin production phase by use of 13C tracers and a complementary platform of analytical techniques.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Susanne Katharina Schwechheimer\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 3736998813\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9783736998810\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Cuvillier\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 11\/26\/2018\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 174\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 0.47lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 8.27h x 5.83w x 0.37d","brand":"Susanne Katharina Schwechheimer","offers":[{"title":"Paperback","offer_id":48446312022271,"sku":"9783736998810","price":59.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_3f107e9b-c613-483f-9051-c9a21ffa60cb.jpg?v=1777223854","url":"https:\/\/www.whiterainbookhouse.com\/products\/riboflavin-production-with-ashbya-gossypii-susanne-katharina-schwechheimer-9783736998810","provider":"WR Book House","version":"1.0","type":"link"}