{"product_id":"synthetic-green-fluorescent-protein-chromophores-jian-dong-9783639134537","title":"Synthetic Green Fluorescent Protein Chromophores","description":"GFP is widely used as a biological marker because of its superb fluorescence properties. We have synthesized GFP chromophore and its derivatives, and their photochemistry and photophysics were investigated using various steady-state and time-resolved techniques. We first compared their optical properties between solution and solid state. We found some O-alkyl GFP chromophore derivatives exhibit large fluorescent enhancement in the solid state. Cis\/trans isomerization is thought to be responsible for blinking behavior in fluorescent protein; however, the mechanism of the thermal reverse isomerization is more problematic. Using Hammett plot studies, we conceived, for the first time, a novel nucleophilic addition\/elimination mechanism. Finally, a hydroxyl substituent at the meta position shows enhanced charge transfer and greater acidity in the excited state. As a result, we have demonstrated that the fast quenching of the excited state by internal conversion to the ground state is much slower in meta- than in para-HOBDI derivatives. This allows studies of this ultrafast intermolecular ESPT that competes with isomerization.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Jian Dong\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 3639134532\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9783639134537\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e VDM Verlag\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 04\/03\/2009\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 128\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 0.44lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 9.00h x 6.00w x 0.30d","brand":"Jian Dong","offers":[{"title":"Paperback","offer_id":47424583041279,"sku":"9783639134537","price":63.72,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_73070399-c137-4ce8-b269-cbedc727a110.jpg?v=1761531193","url":"https:\/\/www.whiterainbookhouse.com\/products\/synthetic-green-fluorescent-protein-chromophores-jian-dong-9783639134537","provider":"WR Book House","version":"1.0","type":"link"}