{"product_id":"synthetic-messenger-rna-and-cell-peter-m-rabinovich-9781493959679","title":"Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols","description":"\u003cp\u003ePart I. mRNA Synthesis, Purification, and Transfer\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e1. Cell Engineering with Synthetic Messenger RNA \u003c\/p\u003e\u003cp\u003e Peter M. Rabinovich and Sherman M. Weissman\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e2. In vitro Transcription of Long RNA Containing Modified Nucleosides \u003c\/p\u003e\u003cp\u003eNorbert Pardi, Hiromi Muramatsu, Drew Weissman, and Katalin Karikó\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e3. HPLC Purification of in vitro Transcribed Long RNA \u003c\/p\u003e\u003cp\u003e Drew Weissman, Norbert Pardi, Hiro Muramatsu, and Katalin Karikó\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e4. Synthetic mRNAs with Superior Translation and Stability Properties\u003c\/p\u003e\u003cp\u003e Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz, Ugur Sahin, Jacek Jemielity, and Robert E. Rhoads\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e5. Non-Viral, Cationic Lipid-Mediated Delivery of mRNA \u003c\/p\u003e\u003cp\u003e James G. Hecker \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e6. Light-Induced mRNA Transfection \u003c\/p\u003e\u003cp\u003e Sigurd Lein s B e and Eivind Hovig\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e7. Engineering B Cells with mRNA \u003c\/p\u003e\u003cp\u003e Jaewoo Lee, David Boczkowski, and Smita Nair\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e8. Programming Human Dendritic Cells with mRNA \u003c\/p\u003e\u003cp\u003e Jaewoo Lee, David Boczkowski, and Smita Nair\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e9. Large Volume Flow Electroporation of mRNA: Clinical Scale Process\u003c\/p\u003e\u003cp\u003e Linhong Li, Cornell Allen, Rama Shivakumar, and Madhusudan V. Peshwa\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e10. Retrovirus-Based mRNA Transfer for Transient Cell Manipulation \u003c\/p\u003e\u003cp\u003e Melanie Galla, Axel Schambach, and Christopher Baum\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e11. mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells\u003c\/p\u003e\u003cp\u003e Frank Breinig, Tanja Breinig, and Manfred J. Schmitt\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart 2. Modulation and monitoring of cell metabolism \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy\u003c\/p\u003e\u003cp\u003e Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors \u003c\/p\u003e\u003cp\u003e Noriko Shimasaki and Dario Campana\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA\u003c\/p\u003e\u003cp\u003e Peizhe Wang and Jie Na\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies\u003c\/p\u003e\u003cp\u003e Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA\u003c\/p\u003e\u003cp\u003e Chantal Pichon and Patrick Midoux\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA \u003c\/p\u003e\u003cp\u003e Özcan Met and Inge Marie Svane\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e18. mRNA electroporation as a Tool for Immunomonitoring\u003c\/p\u003e\u003cp\u003e Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e19. mRNA PCR-Based Epitope Chase Method \u003c\/p\u003e\u003cp\u003e Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePart 2. Modulation and monitoring of cell metabolism \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy\u003c\/p\u003e\u003cp\u003e Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors \u003c\/p\u003e\u003cp\u003e Noriko Shimasaki and Dario Campana\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA\u003c\/p\u003e\u003cp\u003e Peizhe Wang and Jie Na\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies\u003c\/p\u003e\u003cp\u003e Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Peter M. Rabinovich\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1493959670\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781493959679\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 08\/23\/2016\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 323\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.29lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.70d","brand":"Peter M. Rabinovich","offers":[{"title":"Paperback","offer_id":44064768721151,"sku":"9781493959679","price":139.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_96045802-e98d-4140-944b-3ff7703f172f.jpg?v=1685397452","url":"https:\/\/www.whiterainbookhouse.com\/products\/synthetic-messenger-rna-and-cell-peter-m-rabinovich-9781493959679","provider":"WR Book House","version":"1.0","type":"link"}