{"product_id":"synthetic-mrna-robert-e-rhoads-9781493980987","title":"Synthetic Mrna: Production, Introduction Into Cells, and Physiological Consequences","description":"\u003cp\u003eThis volume presents detailed laboratory protocols for \u003ci\u003ein vitro\u003c\/i\u003e synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003ci\u003e\u003cbr\u003eSynthetic mRNA: Production, Introduction into Cells, and Physiological Consequences \u003c\/i\u003eis a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology. \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Robert E. Rhoads\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 149398098X\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781493980987\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 05\/26\/2018\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 320\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.28lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.70d","brand":"Robert E. Rhoads","offers":[{"title":"Paperback","offer_id":43985850237183,"sku":"9781493980987","price":119.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_e9ac3feb-5820-4fc4-b027-2888350a521c.jpg?v=1683279907","url":"https:\/\/www.whiterainbookhouse.com\/products\/synthetic-mrna-robert-e-rhoads-9781493980987","provider":"WR Book House","version":"1.0","type":"link"}