{"product_id":"targeted-protein-degradation-angela-m-cacace-9781071616642","title":"Targeted Protein Degradation: Methods and Protocols","description":"\u003cp\u003ePart I Methods to Discover and Characterize Ligands for Targets and\/or Ligases.\u003c\/p\u003e \u003cp\u003e1. Protein Ligand Interactions Using Surface Plasmon Resonance \u003c\/p\u003e \u003cp\u003eNichole O'Connell \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e2. High-Throughput Detection of Ligand-Protein Binding using a SplitLuc Cellular Thermal Shift Assay \u003c\/p\u003e \u003cp\u003eTino W. Sanchez, Ashley Owens, Natalia J. Martinez, Eric Wallgren, Anton Simeonov, and Mark J. Henderson\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e3. Proteins and their Interacting Partners: An Introduction to Protein-Ligand Binding Site Prediction Methods with a Focus on FunFOLD3 \u003c\/p\u003e \u003cp\u003eDanielle Allison Brackenridge and Liam James McGuffin\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e4. Evaluating ligands for ubiquitin ligases using affinity beads\u003c\/p\u003e\u003cp\u003eJennifer Dobrodziej, Hanqing Dong, Kurt Zimmermann, and Christopher M. Hickey\u003c\/p\u003e \u003cp\u003ePart II Approaches to Study Ligase: Degrader: Target Ternary Complexes.\u003c\/p\u003e 5. Mechanistic and Structural Features of PROTAC Ternary Complexes \u003cp\u003e\u003c\/p\u003e \u003cp\u003eRyan Casement, Adam Bond, Conner Craigon, and Alessio Ciulli \u003c\/p\u003e \u003cp\u003e\u003c\/p\u003e \u003cp\u003e6. MST and TRIC Technology to Reliably Study PROTAC Binary and Ternary Binding In Drug Development \u003c\/p\u003e Tanja Bortoschik, Andreas Zoephel, Klaus Rumpel, Alessio Ciulli, and Charles Heffern\u003cp\u003e\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e7. An in vitro Pull-down Assay of the E3 ligase: PROTAC: Substrate Ternary Complex to Identify Effective PROTACs\u003c\/p\u003e \u003cp\u003eDaniel P. Bondeson, Blake E. Smith, and Alexandru D. Buhimschi\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e8. Kinetic detection of E3: PROTAC: Target Ternary Complexes using NanoBRET technology in Live Cells \u003c\/p\u003e \u003cp\u003eSarah D. Mahan, Kristin M. Riching, Marjeta Urh, and Danette L. Daniels\u003c\/p\u003e \u003cp\u003ePart III Approaches and Tools to Understand Ubiquitylation and Proteasomal Degradation\u003c\/p\u003e \u003cp\u003e9. Inducing Ubiquitylation and Protein Degradation as a Drug Development Strategy \u003c\/p\u003e \u003cp\u003eKamaldeep S. Dhami and XiaoDong Huang\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e10. Methodological Versatility of Tandem Ubiquitin Binding Entities (TUBEs) for Understanding Poly-ubiquitination and its Various Chains \u003c\/p\u003e \u003cp\u003eKatie J. Sheets, Patrick H. Gross, Myra J. Zerr, and Dahmane Ouazia, and Karteek Kadimisetty \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e 11. Global Mass Spectrometry-Based Analysis of Protein Ubiquitination Using K-e-GG Remnant Antibody Enrichment \u003cp\u003e\u003c\/p\u003e Alissa J. Nelson, Yiying Zhu, Jian Min Ren, and Matthew P. Stokes\u003cp\u003e\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e12. Determination of Proteasomal Unfolding Ability \u003c\/p\u003e \u003cp\u003eby Christina M. Hurley and Daniel A. Kraut \u003c\/p\u003e \u003cp\u003ePart IV Methods for Evaluating Protein Degrader Function \u003c\/p\u003e \u003cp\u003e13. Methods for Quantitative Assessment of Protein Degradation \u003c\/p\u003e \u003cp\u003eRadoslaw P. Nowak, Hong Yue, Emily Y. Park, and Eric S. Fischer\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e 14. A High Throughput method to prioritize PROTAC intracellular target engagement and cell permeability using NanoBRET \u003cp\u003e\u003c\/p\u003e \u003cp\u003eby James D. Vasta, Cesear R. Corona, and Matthew B. Robers\u003c\/p\u003e \u003cp\u003e\u003c\/p\u003e \u003cp\u003e15. Profiling CELMoD Mediated Degradation of Cereblon Neosubstrates\u003c\/p\u003e \u003cp\u003eby Joel W. Thompson, Thomas Clayton, Gody Khambatta, Leslie A. Bateman, Christopher W. Carroll, Philip P. Chamberlain, and Mary E. Matyskiela\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e16. Global Proteome Profiling to Assess Changes in Protein Abundance using I\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Angela M. Cacace\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 1071616641\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781071616642\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 08\/26\/2021\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 351\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.80lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.20h x 8.20w x 0.90d\u003c\/p\u003e","brand":"Angela M. 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