{"product_id":"the-crac-channel-aubin-penna-9781493987023","title":"The Crac Channel: Methods and Protocols","description":"This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM\/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cp\u003e\u003c\/p\u003eThorough and cutting-edge, \u003ci\u003eThe CRAC Channel: Methods and Protocols\u003c\/i\u003e is a valuable resource for any researcher interested in learningmore about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Aubin Penna\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 149398702X\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9781493987023\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Humana\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 09\/10\/2018\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 204\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 1.32lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 10.00h x 7.00w x 0.56d","brand":"Aubin Penna","offers":[{"title":"Hardcover","offer_id":47419513209087,"sku":"9781493987023","price":109.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_4915d327-a8dc-4895-9653-fba985d448eb.jpg?v=1761512011","url":"https:\/\/www.whiterainbookhouse.com\/products\/the-crac-channel-aubin-penna-9781493987023","provider":"WR Book House","version":"1.0","type":"link"}