{"product_id":"the-immunological-synapse-part-c-clment-thomas-9780443218682","title":"The Immunological Synapse Part C: Volume 193","description":"\u003ci\u003eThe Immunological Synapse - Part C, \u003c\/i\u003eVolume 193 in the Methods in Cell Biology series provides state-of-the-art and detailed methods for the study of the T cell, natural killer (NK) cell and B cell immunological synapses. Topics covered in this third volume include tau-STED microscopy for 3D-quantitative colocalization of lytic granule markers, image processing approaches for 3D-analysis of markers and for microtubule remodeling quantification at the immunological synapse, flow cytometry to analyze NK cell-target cell conjugation, imaging flow cytometry to quantify a range of different subcellular processes at the immunological synapse, imaging techniques aimed at quantitatively analyzing B cell immunological synapse formation, quantification of force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors, measuring interaction forces between T lymphocytes and their target cells using live microscopy and laminar shear flow chambers, measuring interaction kinetics between T cells and their target tumor cells with optical tweezers, and gauging antigen recognition by human primary T-cells featuring orthotopically exchanged TCRs of choice. Additionally, this volume includes methods to isolate and characterize primary NK cells and enrich the KIR2DL1+ population, and to expand γδ T cells and evaluate their cytotoxicity.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e Cl?ment Thomas\u003cbr\u003e\u003cb\u003eISBN-10:\u003c\/b\u003e 0443218684\u003cbr\u003e\u003cb\u003eISBN-13:\u003c\/b\u003e 9780443218682\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e Academic Press\u003cbr\u003e\u003cb\u003eLanguage:\u003c\/b\u003e English\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 02\/04\/2025\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 280\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e Hardcover","brand":"Cl?ment Thomas","offers":[{"title":"Hardcover","offer_id":46685810622719,"sku":"9780443218682","price":175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0662\/2982\/9887\/files\/img_fd6c75fd-3e2c-4be9-be8a-7912321dfaf2.jpg?v=1741726841","url":"https:\/\/www.whiterainbookhouse.com\/products\/the-immunological-synapse-part-c-clment-thomas-9780443218682","provider":"WR Book House","version":"1.0","type":"link"}