Applications of Genome Modulation and Editing (Hardcover) (ISBN-13: 9781071623008)

Part I: Gene Editing Approaches

1. Historical DNA manipulation Overview

LLuis Montoliu

2. Tools for efficient genome editing; ZFN, TALEN, and CRISPR

Yasaman Shamshirgaran, ^, Jun Liu, Huseyin Sumer, Paul J. Verma and Amir Taheri-Ghahfarokhi

Part II: Genetic Manipulation Methods

3. Efficient generation of stable cell lines with inducible neuronal transgene expression using the piggyBac transposon system

Jinchao Gu, Huseyin Sumer and Brett Cromer

4. Modifying Bacterial Artificial Chromosomes for Extended Genome Modification Hannah Auch, Nikolai Klymiuk and Petra Runa-Vochozkova

5. Site specific editing of the genome in Pluripotent cells Luis Ortega and Joseph Rosenbluh

6. Targeting the AAVS1 site with transgene cassette by CRISPR/Cas9 with an inducible transgene cassette for the to neuronal differentiation of human pluripotent stem cells

Jinchao Gu, Ben Rollo, Huseyin Sumer and Brett Cromer

7. Microinjection of zygotes for CRISPR/Cas9 mediated insertion of transgenes into the murine Rosa26 safe harbour

Fabien Delerue and Lars M. Ittner

8. CRISPR-on for endogenous activation of SMARCA4 expression in bovine embryos

Virgilia Alberio, Virginia Savy and Daniel F. Salamone

Part III: Applications of Genome Manipulation

9. CRISPR/Cas mutagenesis to generate novel traits in Bactrocera tryoni for Sterile Insect Technique

Amanda Choo, Elisabeth Fung, Thu N.M. Nguyen, Anzu Okada and Peter Crisp

10. CRISPR/Cas9 Genome Editing in the New World Screwworm and Australian Sheep Blowfly

Daniel F. Paulo, Megan E. Williamson and Maxwell J. Scott

11. Generation of gene drive mice for invasive pest population suppression

Mark Bunting, Chandran Pfitzner, Luke Gierus, Melissa White, Sandra Piltz and Paul Thomas

Part IV: Large animal models of human disease

12. Generation of a human deafness sheep model using the CRISPR/Cas system

Martina Crispo, Vanessa Chenouard, Pedro dos Santos-Neto, Laurent Tesson, Marcela Souza, JeanMarie Heslan, Federico Cuadro, Ignacio Aneg and Alejo Menchaca

13. Targeted Gene Editing in Porcine Germ Cells

Taylor Goldsmith, Alla Bondareva, Dennis Webster, Anna Laura Voigt, Lin Su, Daniel F. Carlson and Ina Dobrinski

14. Generating a Heat Tolerance Mouse Model

Jun Liu and Paul J. Verma

Part V: Large Animal Welfare and Production Outcomes

15. Generation of pigs that produce single sex progeny

Bjn Petersen and Stefanie Kurtz

16.

Author: Paul John Verma
ISBN-10: 1071623001
ISBN-13: 9781071623008
Publisher: Humana
Language: English
Published: 06/14/2022
Pages: 369
Format: Hardcover
Weight: 1.94lbs
Size: 10.00h x 7.00w x 0.88d