Chondrocytes: Methods and Protocols (Hardcover) (ISBN-13: 9781071611180)

1. Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D

Rodrigo A. Somoza and Jean F. Welter

2. Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cartilage

Ilona Uzieliene, Jaroslav Denkovskij, Eiva Bernotiene, Ursule Kalvaityte, Raminta Vaiciuleviciute, Yolande F.M. Ramos, and Ali Mobasheri

3. Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse

Ioannis Kanakis, Mohammad Alhashmi, Ke Liu, Craig Keenan, Lorenzo Ramos Mucci, Blandine Poulet, and George Bou-Gharios

4. Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures

Abdul Haseeb and V駻onique Lefebvre

5. Fetal Growth Plate Cartilage: Histological and Immunohistochemical Techniques

Zachary Tata, Christophe Merceron, and Ernestina Schipani

6. Preparation of Adult Mouse Skeletal Tissue Sections for RNA In Situ Hybridization

Charles de Charleroy, Abdul Haseeb, and V駻onique Lefebvre

7. MicroRNA In Situ Hybridization in Paraffin-Embedded Human Articular Cartilage and Mouse Knee Joints

Helal Endisha and Mohit Kapoor

8. Laser Capture Microdissection of Mouse Growth Plate Cartilage

Bijal Kikani and Julian C. Lui

9. RNA Isolation from Articular Cartilage Tissue

Hongjun Zheng and Audrey McAlinden

10. Small Nucleolar RNA Expression Profiling in Cartilage

Mandy J. Peffers, Andy Cremers, and Tim J.M. Welting

11. MicroRNA Expression Profiling, Target Identification, and Validation in Chondrocytes

Tomoki Chiba, Ryota Kurimoto, Takahide Matsushima, Yoshiaki Ito, Ryo Nakamichi, Martin Lotz, and Hiroshi Asahara

12. ChIP-Seq Assays from Mammalian Cartilage and Chondrocytes

Akira Yamakawa, Hironori Hojo, and Shinsuke Ohba

13. MicroRNAs and Regulation of Autophagy in Chondrocytes

Yolande F.M. Ramos and Ali Mobasheri

14. Mitochondrial Biogenesis, Activity, and DNA Isolation in Chondrocytes

Yun Wang, Liang-Yu Chen, and Ru Liu-Bryan

15. Assessing Chondrocyte Status by Immunofluorescence-Mediated Localization of Parkin Relative to Mitochondria