Directed Evolution: Methods and Protocols (Hardcover) (ISBN-13: 9781071621516)

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

Author: Andrew Currin
ISBN-10: 1071621513
ISBN-13: 9781071621516
Publisher: Humana
Language: English
Published: 06/22/2022
Pages: 278
Format: Hardcover
Weight: 1.60lbs
Size: 10.00h x 7.00w x 0.69d