DNA Vaccines: Methods and Protocols (Paperback) (ISBN-13: 9781071608746)

Part I: Vector design For vaccination

1. A Novel Cre Recombinase Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary Application

Yanlong Jiang, Guilian Yang, and Chunfeng Wang

2. Vaccination with Messenger RNA - a Promising Alternative to DNA Vaccination

Istv疣 Tomb當z, Drew Weissman, and Norbert Pardi

3. Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency

Kenneth Lundstrom

Part II: DNA Vaccine Adjuvants and Immunostimulatory Response

4. CpG Oligonucleotides as Vaccine Adjuvants

Neslihan Kayraklioglu, Begum Horuluoglu, and Dennis M. Klinman

5. Molecular Adjuvants for DNA Vaccines: Application, Design, Preparation and Formulation

Ailar Sabbaghi and Amir Ghaemi

6. Assessing Antigen-specific Cellular Immune Responses Upon HIV/SIV Plasmid DNA Vaccination in the Non-human Primate Model

Xintao Hu, Barbara K. Felber, and Antonio Valentin

Part III Biotechnological Processes to Obtain DNA Vaccines

7. Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design

Lu﨎 A. Passarinha

8. Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation

Alexandra Wagner, A. Rita Silva-Santos, Sara Sousa Rosa, Sophie Gierak, Ana M. Azevedo, and Duarte Miguel F. Prazeres

9. Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic Monoliths

Urh Černigoj and Ales Strancar

10. Purification of Plasmid DNA by Multimodal Chromatography

A. Rita Silva-Santos, Sara Sousa Rosa, Duarte Miguel F. Prazeres, and Ana M. Azevedo

11. Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment

Ana M. Almeida, Dalinda Eus饕io, Jo縊 A. Queiroz, Fani Sousa, and ﾂngela Sousa

Part IV: DNA Vaccine Delivery

12. Tumor-Specific CD8⁺ T Cell Responses Induced by DNA Vaccination

Pablo C當eres-Morgado and Alvaro Lladser

13. Therapeutic DNA Vaccine Against HPV16-Associated Cancer

Meihua Yu and Janin Chandra

14. Bulk and Microfluidic Synthesis of Stealth and Cationic-Liposomes for Gene Delivery Applications

Lucimara Gaziola de la Torre, Amanda da Costa e Silva de Noronha Pessoa, Bruna Gregatti de Carvalho, Thiago Bezerra Taketa, Ismail Es, and Gabriel Perli

15. Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field

Diana Costa, ﾂngela Sousa, R炻en Faria, Ana Raquel Neves, and Jo縊 A. Queiroz

16. Main Features of DNA Based Vectors for Use in Lactic Acid Bacteria and Update Protocols

Nina D. Coelho-Rocha, Fernanda A. L. Barroso, La﨎a M. Tavares, Ester S. S. dos Santos, Vasco Azevedo, Mariana M. Drumond, and Pamela Mancha-Agresti

Part V: DNA Vaccine Transfer to Clinical Trials

17. Ethics of DNA Vaccines Transfer for Clinical Research

Ana Cristina Ramalhinho and Miguel Castelo-Branco

18. Preparation of an Academic Clinical TrialPreparation of an academic clinical trial

Ana Cristina Ramalhinho and Miguel Castelo-Branco

Author: ﾂngela Sousa
ISBN-10: 1071608746
ISBN-13: 9781071608746
Publisher: Humana
Language: English
Published: 08/22/2021
Pages: 335
Format: Paperback
Weight: 1.33lbs
Size: 10.00h x 7.00w x 0.72d