Fungal Genomics: Methods and Protocols (Hardcover) (ISBN-13: 9781617790393)

1. Genome Sequencing and Assembly

Manfred G. Grabherr, Evan Mauceli, and Li-Jun Ma

2. Targeted Cloning of Fungal Telomeres

Mark L. Farman

3. Identification and Annotation of Repetitive Sequences in Fungal Genomes

Braham Dhillon and Stephen B. Goodwin

4. Next-Generation Sequencing and Potential Applications in Fungal Genomics

Phillip SanMiguel

5. Getting the Most Out of Your Fungal Microarray Data: Two Cost and Time-Effective Methods

Sandra M. Mathioni, André Beló, Jeffrey P. Townsend, and Nicole M. Donofrio

6. Fusarium graminearum from Expression Analysis to Functional Assays

Heather E. Hallen-Adams, Brad L. Cavinder, and Frances Trail

7. EST Analysis Pipeline: Use of Distributed Computing Resources

Francisco Javier González and Juan Antonio Vizcaíno

8. The Application of ChIP-chip Analysis in the Rice Blast Pathogen

Soonok Kim and Thomas Mitchell

9. Proteome Studies of Filamentous Fungi

Scott E. Baker and Ellen A. Panisko

10. A Bioinformatics Pipeline for Sequence-Based Analyses of Fungal Biodiversity

D. Lee Taylor and Shawn Huston

11. Identifying Protein Complexes by Affinity Purification and Mass Spectrometry Analysis in the Rice Blast Fungus

Wende Liu, Anton Iliuk, Andy Tao, and Shengli Ding

12. Large Scale Identification of Genes Involved in Plant-Fungal Interactions Using Illumina's Sequencing-By-Synthesis Technology

R. C. Venu, Yuan Zhang, Brian Weaver, Peter Carswell, Thomas K. Mitchell, Blake C. Meyers, Michael J. Boehm, and Guo-Liang Wang

13. High-Throughput Production of Gene Replacement Mutants in Neurospora crassa

Gyungsoon Park, Hildur V. Colot, Patrick D. Collopy, Svetlana Krystofova, Christopher Crew, Carol Ringelberg, Liubov Litvinkova, Lorena Altamirano, Liande Li, Susan Curilla, Wei Wang, Norma Gorrochotegui-Escalante, Jay C. Dunlap, and Katherine A. Borkovich

14. Phenotypic Analysis of Neurospora crassa Gene Deletion Strains

Gloria E. Turner

15. Efficient Approaches for Generating GFP Fusion and Epitope-Tagging Constructs in Filamentous Fungi

Xiaoying Zhou, Guotian Li, and Jin-Rong Xu

16. Large Scale Insertional Mutagenesis in Magnaporthe oryzae by Agrobacterium tumefaciens-Mediated Transformation

Xiao-Lin Chen, Jun Yang, and You-Liang Peng

17. Molecular Methods for Studying the Cryphonectria parasitica - Hypovirus Experimental System

Angus L. Dawe, Rong Mu, Gloricelys Rivera, and Joanna A. Salamon

18. Metabolic Fingerprinting in Fusarium verticillioides to Determine Gene Function

Jonathon E. Smith and Burton H. Bluhm

19. Tapping Genomics to Unravel Ectomycorrhizal Symbiosis

Jonathan M. Plett, Barbara Montanini, Annegret Kohler, Simone Ottonello, and Francis Martin