Heterologous Expression of Membrane Proteins: Methods and Protocols (Hardcover) (ISBN-13: 9781071623671)

1. Solubilization and Purification of a₅b₁ Integrin from Rat Liver for Reconstitution into Nanodiscs

Estelle Dransart, Aur駘ie Di Cicco, Ahmed El Marjou, Daniel L騅y, Staffan Johansson, Ludger Johannes, and Massiullah Shafaq-Zadah

2. Membrane Proteins Production in Escherichia coli: Protocols and Rules

Jordi Royes, Pauline Talbot, Christel Le Bon, Karine Moncoq, Marc Uzan, Francesca Zito, and Bruno Miroux

3. Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly

Margot Di Cesare, Aissatou Maty Diagne, Benjamin Bourgey, Jean-Michel Jault, and C馘ric Orelle

4. Heterologous Expression of Membrane Proteins in E. coli

Peer Depping, Mar? Monserrat Rom疣 Lara, Athanasios Kesidis, Roslyn M. Bill, Alice J. Rothnie, Douglas F. Browning, and Alan D. Goddard

5. Overexpression of the ABC Transporter BmrA within Intracellular Caveol in Escherichia coli

Alexia Gobet, Christine Jaxel, Sandrine Magnard, Manuel Garrigos, St駱hane Orlowski, Nad鑒e Jamin, Pierre Falson, and Vincent Chaptal

6. Production of Membrane Proteins in Pseudomonas stutzeri

Hao Xie, Cornelia Muenke, Manuel Sommer, Sabine Buschmann, and Hartmut Michel

7. Overproduction of Membrane-Associated, and Integrated, Proteins Using Saccharomyces cerevisiae

Landon Haslem, Marina Brown, Xin A. Zhang, Jennifer M. Hays, and Franklin A. Hays

8. Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae

Landon Haslem, Marina Brown, Xin A. Zhang, Jennifer M. Hays, and Franklin A. Hays

9. Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p

Jorgaq Pata, Alexis Moreno, Sandrine Magnard, Atanu Banerjee, Rajendra Prasad, and Pierre Falson

10. Membrane Protein Production in the Yeast, P. pastoris

Hoor Ayub, Michelle Clare, Luke Broadbent, John Simms, Alan D. Goddard, Alice J. Rothnie, and Roslyn M. Bill

11. Production and Preparation of Isotopically-Labelled Human Membrane Proteins in Pichia pastoris for Fast-MAS-NMR Analyses

Lina Barret, Tobias Schubeis, Val駻ie Kugler, Lucile Guyot, Guido Pintacuda, and Renaud Wagner

12. Membrane Protein Production in Insect Cells

Afroditi Vaitsopoulou, Peer Depping, Roslyn M. Bill, Alan D. Goddard, and Alice J. Rothnie

13. Thromboxane A₂ G Protein-Coupled Receptor Production and Crystallization for Structure Studies

Pawel Krawinski and Martin Caffrey

14. Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies

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Author: Isabelle Mus-Veteau
ISBN-10: 1071623672
ISBN-13: 9781071623671
Publisher: Humana
Language: English
Published: 07/01/2022
Pages: 467
Format: Hardcover
Weight: 2.33lbs
Size: 10.00h x 7.00w x 1.06d