Membrane Trafficking: Methods and Protocols (Hardcover) (ISBN-13: 9781071622087)

Part I: The Exocytic Pathway

1. Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System

Marine D. Camus and Stephane M. Camus

2. Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo

Julia Dancourt and Gr馮ory Lavieu

3. Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH)

Cedric S. Asensio

4. Spatial and Temporal Control of Protein Secretion with Light

Ashley M. Bourke and Matthew J. Kennedy

5. Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing

Syed Mukhtar Ahmed

6. Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting

Kunrong Mei, Di-Ao Liu, and Wei Guo

7. Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy

Fei Kang and Herbert Y. Gaisano

8. Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique

Jihong Gong, Xiaofei Yang, and Cong Ma

9. Live-Cell Super-Resolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons

Tong Wang and Fr馘駻ic A. Meunier

10. Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse

Tamara Basta, Garry P. Morgan, Eileen T. O'Toole, Nalini R. Rao, Jeffrey N. Savas, and Michael H.B. Stowell

11. In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion

Yinghui Liu, Ruyue He, Min Zhu, and Haijia Yu

12. Imaging Single Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)

Part II: The Endocytic Pathway

13. Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy

Bijeta Prasai and Justin W. Taraska

14. Endocytosis Assays Using Cleavable Fluorescent Dyes

Shifeng Wang, Chun Wan, Galen T. Squiers, and Jingshi Shen

15. Reconstituting and Purifying Assembly Intermediates of Clathrin Adaptors AP1 and AP2

Bing Wang, Rui Yang, Yuan Tian, and Qian Yin

16. Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches

Amulya Priya and Sunand

Author: Jingshi Shen
ISBN-10: 1071622080
ISBN-13: 9781071622087
Publisher: Humana
Language: English
Published: 07/13/2022
Pages: 407
Format: Hardcover
Weight: 2.09lbs
Size: 10.00h x 7.00w x 0.94d