Metabolic engineering of Basfia succiniciproducens for the production of carbon-three compounds (Band 7) (Paperback) (ISBN-13: 9783736999138)

Beschreibung The recent rise of bio-succinic acid as an industrial platform chemical has brought the rumen bacterium Basfia succiniciproducens, a natural producer of this organic acid, into the focus of research. This work investigated the potential of B. succiniciproducens for the production of carbon-three chemicals from sugars. Beside the optimization of improved genetic tools, the C3 chemicals alanine, β-alanine and 3-hydroxypropionate (3-HP) were targeted. A new alanine producer, B. succiniciproducens ALA-1, exhibited good performance in the bioprocess. The next step demonstrated the proof-of-concept for β-alanine synthesis using the heterologous expression of aspartate 1 decarboxylase genes from Corynebacterium glutamicum or Vibrio natriegens. The expression of two additional genes enabled the de novo synthesis of up to 100 mg L-1 3-HP from glucose.

Author: Jonathan Thomas Fabarius
ISBN-10: 3736999135
ISBN-13: 9783736999138
Publisher: Cuvillier
Language: English
Published: 12/06/2018
Pages: 144
Format: Paperback
Weight: 0.40lbs
Size: 8.27h x 5.83w x 0.31d