Mouse Models of Allergic Disease: Methods and Protocols (Hardcover) (ISBN-13: 9781627034951)

1. Transgenic Mouse Models

Jennifer Skelton, Linda H. Gower, Anne McGough, and Patricia A. Labosky

2. Pulmonary Antigen Presenting Cells: Isolation, Purification, and Culture

Hideki Nakano and Donald N. Cook

3. Evaluation of T Cell Function in Allergic Disease

Brianne R. Barker

4. Evaluating B-Cells: From Bone Marrow Precursors to Antibody Producing Cells

M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, and Rita Carsetti

5. Protocols for Identifying, Enumerating and Assessing Mouse Eosinophils

Kimberly D. Dyer, Katia E. Garcia-Crespo, Caroline M. Percopo, Eva M. Sturm, and Helene F. Rosenberg

6. Evaluation of Classical, Alternative, and Regulatory Functions of Bone Marrow-Derived Macrophages

Beckley K. Davis

7. Applications of Mouse Airway Epithelial Cell Culture for Asthma Research

Amjad Horani, John D. Dickinson, and Steven L. Brody

8. Isolation and Characterization of Mast Cells in Mouse Models of Allergic Diseases

Martina Kovarova

9. Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE

Jamie L. Sturgill and Daniel H. Conrad

10. Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice

Elizabeth Doyle, Julia Trosien, and Martin Metz

11. Contact Hypersensitivity Models in Mice

Irving C. Allen

12. Induction of Allergic Rhinitis in Mice

Virginia McMillan Carr and Alan M. Robinson

13. Induction of Allergic Airway Disease Using House Dust Mite Allergen

Irving C. Allen

14. An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airways Disease

Jane M. Schuh and Scott A. Hoselton

15. PAMPs and DAMPs in Allergy Exacerbation Models

Monique A.M. Willart, Philippe Poulliot, Bart N. Lambrecht, and Mirjam Kool

16. Assessment of Airway Hyperresponsiveness in Mouse Models of Allergic Lung Disease Using Detailed Measurements of Respiratory Mechanics

John M. Hartneyand Annette Robichaud

17. Bilateral Vagotomy as a Tool for Determining Autonomic Involvement in Airway Responses in Mouse Models of Asthma

Jaime M. Cyphert

18. Clara Epithelial Cell Depletion in the Lung

Sanchaita S. Sonar and Jan C. Dudda

19. A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma

Matthias Schmidt and Sabrina Mattoli

20. Assessment of Airway Hyperresponsiveness in Murine Tracheal Rings

Jeremiah T. Herlihy, Iurii Semenov, and Robert Brenner

21. Use of the Cockroach Antigen Model of Acute Asthma to Determine the Immunomodulatory Role of Early Exposure to Gastrointestinal Infection

Carolyn G. Durham, Lisa M. Schwiebert, and Robin G. Lorenz

22. Expression Profiling to Identify Candidate Genes Associated with Allergic Phenotypes

Willie June Brickey

23. Flow Cytometric Methods for the Assessment of Allergic Disease

Adeeb H. Rahman

24. Generation of Bone Marrow and Fetal Liver Chimeric Mice

Eda K. Holl