Mycobacteria Protocols (Paperback) (ISBN-13: 9781071614624)

1. Culturing Mycobacteria

Elizabeth Wallace, Debra Hendrickson, Nicholas Tolli, Carolina Mehaffy, María Peña, Jerry A. Nick, Phillip Knabenbaur, Jackson Watkins, Anne Simpson, Anita G. Amin, Delphi Chatterjee, Karen M. Dobos, Ramanuj Lahiri, Linda Adams, Michael Strong; Max Salfinger, Rebecca Bradford, Timothy T. Stedman, Marco A. Riojas, and Manzour Hernando Hazbón

2. DNA Isolation from Mycobacteria

Heena Jagatia and Daire Cantillon

3. Extraction and Separation of Mycobacterial Proteins

Megan Lucas, Joan M. Ryan, Jackson Watkins, Kala Early, Nicole A. Kruh-Garcia, Carolina Mehaffy, and Karen M. Dobos

4. Lipid and Lipoarabinomannan Isolation and Characterization

Marie-Antoinette Lanéelle, Lucie Spina, Jérôme Nigou, Anne Lemassu, and Mamadou Daffé

5. Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria

Yunlong Li, Pooja Keshavan, Jamie H. Corro, Ravi K. Koripella, Rajendra K. Agrawal, and Anil K. Ojha

6. Macrophage Infection Models for Mycobacterium tuberculosis

7. Novel Single-Cell and High Throughput Microscopy Techniques to Monitor Dictyostelium discoideum-Mycobacterium marinum Infection Dynamics

Manon Mottet, Cristina Bosmani, Nabil Hanna, Jahn Nitschke, Louise Lefrancois, and Thierry Soldati

8. Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy

Giulia Manina and Neeraj Dhar

9. Measuring Efflux and Permeability in Mycobacteria

Liliana Rodrigues, José A. Aínsa, and Miguel Viveiros

10. Drug Susceptibility Screening Using In Vitro Models of Hypoxic Non-Replicating Persistent Mycobacteria

Savannah E.R. Gibson, James Harrison, and Jonathan A.G. Cox

11. Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells

Sydney L. Solomon and Bryan D. Bryson

12. Electroporation of Mycobacteria

Tanya Parish

13. Gene Switching and Essentiality Testing

Amanda Claire Brown

14. Oligo-Mediated Recombineering and Its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis

Kenan C. Murphy

15. Using Proteolytic Hypomorphs to Detect Small Molecule Mechanism of Action

Eachan O. Johnson and Deborah T. Hung

16. CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria

An

Author: Tanya Parish
ISBN-10: 1071614622
ISBN-13: 9781071614624
Publisher: Humana
Language: English
Published: 07/08/2022
Pages: 736
Format: Paperback
Weight: 2.83lbs
Size: 10.00h x 7.00w x 1.50d