Nucleic Acid Aptamers: Selection, Characterization, and Application (Hardcover) (ISBN-13: 9781071626948)

Part I: Selection

1. Automated ssDNA SELEX Using Histidine Tagged Target Proteins

Stefan Breuers and Günter Mayer

2. Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection

Nicole Williams, Rutika Patel, and Prabodhika Mallikaratchy

3. Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX

Moujab Choukeife, Anna Jonczyk, and Günter Mayer

4. Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction

Magda Puchala, Marta Radzińska, Joanna Guzdek, Agnieszka Sok-Grochowska, Aleksandra Adamowicz-Skrzypkowska, Pawel Pięta, Przemyslaw Jurek, and Monika Czarnecka

5. RNA Capture-SELEX on Streptavidin Magnetic Beads

Leon Kraus and Beatrix Suess

6. The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE

Jan Hoinka and Teresa M. Przytycka

7. Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX

Frédéric Ducongé

Part II: Characterization

8. Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry

9. Determination of Aptamer Structure Using Circular Dichroism Spectroscopy

Yannick Kerler, Stephan Sass, Carsten Hille, and Marcus M. Menger

10. Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis

Wiebke Sabrowski, Walter F.M. Stöcklein, and Marcus M. Menger

11. Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance

12. Probing of Fluorogenic RNA Aptamers via Supramolecular Förster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog

Christian Steinmetzger and Claudia Höbartner

13. Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT

Sebastian Strauss and Ralf Jungmann

Part III: Application

14. Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environments

Nako Nakatsuka

15. Aptasensor for Impedimetric Detection of Lysozyme

Ece Eksin, Huseyin Senturk, and Arzum Erdem

16. Biophysical Analysis of Small Molecule Binding to Viral RNA Structures

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Author: Günter Mayer
ISBN-10: 1071626949
ISBN-13: 9781071626948
Publisher: Humana
Language: English
Published: 09/27/2022
Pages: 282
Format: Hardcover
Weight: 1.61lbs
Size: 10.00h x 7.00w x 0.69d