Oligonucleotide Synthesis: Methods and Applications (Hardcover) (ISBN-13: 9781588292339)

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

Author: Piet Herdewijn
ISBN-10: 1588292339
ISBN-13: 9781588292339
Publisher: Humana
Language: English
Published: 09/01/2004
Pages: 436
Format: Hardcover
Weight: 1.87lbs
Size: 9.14h x 14.36w x 1.17d

Review Citation(s):
Scitech Book News 12/01/2004 pg. 73