Protein Electrophoresis: Methods and Protocols (Paperback) (ISBN-13: 9781493962419)

1. How It All Began -- A Personal History of Gel-Electrophoresis

Oliver Smithies

2. Introduction to Protein Electrophoresis

Pothur R. Srinivas

3. Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis

J.P. Dean Goldring

4. Protein Solubilization: Attend to the Choice of Lysis Buffer

Mandy Peach, Noelle Marsh, and Daniel J. MacPhee

5. Native Polyacrylamide Gels

Claudia Arndt, Stefanie Koristka, Holger Bartsch, and Michael Bachmann

6. Cationic Electrophoresis

Engelbert Buxbaum

7. SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins

Christian Reichel

8. Tricine-SDS-PAGE

Syed R. Haider, Helen J. Reid, and Barry L. Sharp

9. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis

Marta P. Pereira Morais, John S. Fossey, Tony D. James, Jean M. H. van den Elsen

10. Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins

Marion L. Greaser and Chad M. Warren

11. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis

Henghang Tsai and Hon-chiu Eastwood Leung

12. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples

Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann

13. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies

Gyorgy Csako

14. Microchip Electrophoresis, with respect to "Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease"

Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy

15. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols

Mustafa Çelebier, Clara Ibáñez, Carolina Simó, and Alejandro Cifuentes

16. Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE

Yassel Ramos, Vladimir Besada, and Lila Castellanos-Serra

17. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass

Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa

18. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting

Edward P. Trieu and Ira N. Targoff

19. Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing

Yaser Dorri

20. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids

Gyorgy Csako

21. Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution

Biji T. Kurien and R. Hal Scofield

22. Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE

Hiroyuki Matsumoto, Hisao Hanju, Biji T. Kurien, and Naoka Komori

23. One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis

Bao-Shiang Lee, G.D. Lasanthi, P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta

24. Two-Dimensional Difference Gel Electrophoresis

Jonathan S. Minden

25. Pro

Author: Biji T. Kurien
ISBN-10: 1493962418
ISBN-13: 9781493962419
Publisher: Humana
Language: English
Published: 08/23/2016
Pages: 648
Format: Paperback
Weight: 2.02lbs
Size: 9.21h x 6.14w x 1.33d