Reactive Oxygen Species in Plants: Methods and Protocols (Hardcover) (ISBN-13: 9781071624685)

Part I: Strategies to Induce ROS Production

1. Modification of Chloroplast Antioxidant Capacity by Plastid Transformation

Shengchun Li, Pan Shen, Bipeng Wang, Xiujie Mu, Mimi Tian, Tao Chen, and Yi Han

2. Analysis of Ascorbate Metabolism in Arabidopsis under High-Light-Stress

Takanori Maruta and Takahiro Ishikawa

3. Genetic Manipulation of Reactive Oxygen Species (ROS) Homeostasis Utilizing CRISPR/Cas9-Based Gene Editing in Rice

Sheng Xu, Tao Chen, Mimi Tian, Marie-Sylviane Rahantaniaina, Linlin Zhang, Ren Wang, Wei Xuan, and Yi Han

4. Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging

Alexey Shapiguzov and Jaakko Kangasjärvi

Part II: Methods to Visualize ROS and to Detect Changes in Redox Homeostasis

5. Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants

José Manuel Ugalde, Lara Fecker, Markus Schwarzländer, Stefanie J. Müller-Schüssele, and Andreas J. Meyer

6. Quantitative Measurement of Ascorbate and Glutathione by Spectrophotometry

Graham Noctor and Amna Mhamdi

7. Measurement of NAD(P)H and NADPH-Generating Enzymes

Amna Mhamdi, Frank Van Breusegem, and Graham Noctor

8. Quantitative Analysis for ROS-Producing Activity and Regulation of Plant NADPH Oxidases in HEK293T Cells

Sachie Kimura, Hidetaka Kaya, Kenji Hashimoto, Michael Wrzaczek, and Kazuyuki Kuchitsu

Part III: Small-Scale Targeted Analysis of ROS Accumulation during Stress and Effects on Plant Physiology

9. Estimation of the Level of Abasic Sites in Plant mRNA Using Aldehyde Reactive Probe

Jagna Chmielowska-Bąk, Karolina Izbiańska-Jankowska, and Joanna Deckert

10. A Simplified Method to Assay Protein Carbonylation by Spectrophotometry

Corentin Moreau and Emmanuelle Issakidis-Bourguet

11. In Vitro Biochemical Analysis of Recombinant Plant Proteins under Oxidation

Zeya Chen and Jingjing Huang

12. Methods to Analyze the Redox Reactivity of Plant Proteins

Thualfeqar Al-Mohanna, George V. Popescu, and Sorina C. Popescu

13. Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids

Brigitte Ksas and Michel Havaux

14. Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed Phase HPLC-MS under Non-Derivatized Conditions

Attila L. Ádám, György Kátay, András Künstler, and Lóránt Király