Recombineering: Methods and Protocols (Hardcover) (ISBN-13: 9781071622322)

Preface...
Table of Contents...
Contributing Authors...

1. Scarless ssDNA Recombineering of Phage in the Lysogenic State
Maria Ababi, Matthew Tridgett, Alexander Osgerby, and Alfonso Jaramillo

2. Lambda red Recombineering of Bacteriophage in the Lysogenic State
Matthew Tridgett, Maria Ababi, and Alfonso Jaramillo

3. Recombineering Mediated Genome Editing in Burkholderiales Strains
Xue Wang, Jiaqi Liu, Wentao Zheng, Youming Zhang, and Xiaoying Bian

4. High-Efficiency Multi-Site Genomic Editing (HEMSE) Made Easy
Tom疽 Aparicio, V?tor de Lorenzo, and Esteban Mart?ez-Garc?

5. Creation of Markerless Genome Modifications in a Non-Model Bacterium by Fluorescence-Aided Recombineering
Piyush Behari Lal, Fritz Wells, and Patricia J. Kiley

6. Plant Gene Modification by BAC Recombineering
Zhoubo Hu, Ajit Ghosh, and Csaba Koncz

7. Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling
Joakim N舖vall

8. Genome Editing in Klebsiella pneumoniae using CRISPR/Cas9 Technology
Zhipeng Wang, Yu Wang, and Quanjiang Ji

9. Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System
Adam J. Ellington and Christopher R. Reisch

10. Recombineering in Staphylococcus aureus
Kelsi Penewit and Stephen J. Salipante

11. Genome Editing Methods for Bacillus subtilis
Katherine J. Wozniak and Lyle A. Simmons

12. Highly Efficient Genome Editing in Clostridium difficile using the CRISPR-Cpf1 System
Wei Hong, Jie Zhang, Guzhen Cui, Qian Zhou, Pixiang Wang, and Yi Wang

13. Genome Editing of Corynebacterium glutamicum using CRISPR-Cpf1 System
Zhiqiang Wen, Fenghui Qian, Jiao Zhang, Yu Jiang, and Sheng Yang

14. CRISPR/Cas-Mediated Genome Editing of Streptomyces
Lee Ling Tana, Elena Heng, Nadiah Zulkarnain, Wan-Chi Hsiao, Fong T. Wonga, and Mingzi M. Zhang

15. Cas9 Nickase-Based Genome Editing in Clostridium cellulolyticum
Tao Xu, Xuanyu Tao, Megan L. Kempher, and Jizhong Zhou

16. Genome Editing with Cas9 in Lactobacilli
Justin M. Vento and Chase L. Beisel

Subject Index List...


Author: Christopher R. Reisch
ISBN-10: 1071622323
ISBN-13: 9781071622322
Publisher: Humana
Language: English
Published: 05/18/2022
Pages: 264
Format: Hardcover
Weight: 1.54lbs
Size: 10.00h x 7.00w x 0.69d