Signal Transduction Immunohistochemistry: Methods and Protocols (Hardcover) (ISBN-13: 9781071628102)

1. Top 5 Considerations When Choosing an Antibody

Amy Archuleta and Michael D. Browning

2. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting

Biji T. Kurien and R. Hal Scofield

3. Recent Advances in Chromogens for Immunohistochemistry

Bipin Gupta, George Yang, Olga Petrauskene, and Marc Key

4. Overview of Imaging Techniques in Immunohistochemistry

Gerald Sedgewick

5. Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells

Pravin J. Mishra and Debabrata Banerjee

6. Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments

Hemant K. Mishra

7. Labeling of Phospho-Specific Antibodies with oYo-Link(R) Epitope Tags for Multiplex Immunostaining

Jingwen Niu, Jodi Hagen, Feifan, Yu, Alexander E. Kalyuzhny, and Andrew Tsourkas

8. Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species

Matthew Levin, Zohreh AkhavanAghdam, and David Schwartz

9. 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes

Sharla L. White, Amy T. Lam, and Hannah D. Buck

10. Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research

Simeon C. Daeschler and Gregory H. Borschel

11. Protocol and Software for Automated Detection of Lysosome Active "Runs" and "Flights" with Wavelet Transform Approach

12. Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing

Christine Goetz, Chris Hammerbeck, Kristina Boss, Ryan Peterson, and Jody Bonnevier

13. In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells

Jodi Hagen, Ashley Oliver, and Alexander E. Kalyuzhny

14. In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue

Ashley Oliver, Jodi Hagen, Sue Yang, and Alexander E. Kalyuzhny

15. Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry

Wenfei Kang, Anthony Santella, Eric Rosiek, Maria Pulina, Eric Chan, Ning Fan, Murray J. Tipping, Afsar Barlas, Yevgeniy Romin, and Katia Manova-Todorova

16. Transcriptio

Author: Alexander E. Kalyuzhny
ISBN-10: 1071628100
ISBN-13: 9781071628102
Publisher: Humana
Language: English
Published: 12/14/2022
Pages: 328
Format: Hardcover
Weight: 1.78lbs
Size: 10.00h x 7.00w x 0.81d