Stem Cells and Tissue Repair: Methods and Protocols (Paperback) (ISBN-13: 9781493955329)

1. Culturing and Differentiating Mouse Embryonic Stem Cells

Axel P. Gross and Chrissa Kioussi

2. Neural Stem Cells Transplantation in an Animal Model of Traumatic Brain Injury

Dimitra Thomaidou

3. Experimental Cell Transplantation for Traumatic Spinal Cord Injury Regeneration: Intramedullar or Intrathecal Administration

Ana Alastrue-Agudo, Slaven Erceg, Marta Cases-Villar, Viviana Bisbal-Velasco, Richard J. Griffeth, Francisco Javier Rodriguez-Jim駭ez, and Victoria Moreno-Manzano

4. Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for in vivo Analysis of the Brain Tumor Initiating Cell

Maleeha Qazi, Aneet Mann, Randy van Ommeren, Chitra Venugopal, Nicole McFarlane, Parvez Vora, and Sheila K. Singh

5. Growth of Bone Marrow and Skeletal Muscle Side Population Stem Cells in Suspension Culture

Christina A. Pacak and Douglas B. Cowan

6. Isolation, Culture and Immunostaining of Skeletal Muscle Fibers to Study Myogenic Progression in Satellite Cells

Louise A. Moyle and Peter S. Zammit

7. Human Neural Crest Stem Cells Derived from Human Pluripotent Stem Cells

Qiuyue Liu, Andrzej Swistowski, and Xianmin Zeng

8. Dental Pulp Stem Cells (DPSC) Isolation, Characterization and Differentiation

Federico Ferro, Renza Spelat, and Chelsea S. Baheney

9. Dental Pulp Stem Cells Isolation and Osteogenic Differentiation: A Good Promise for Tissue Engineering

Adriana Di Benedetto, Claudia Carbone, and Giorgio Mori

10. Efficient Hepatic Differentiation of Human Induced Pluripotent Stem Cells in A Three-Dimensional Microscale Culture

Ran-Ran Zhang, Takanori Takebe, Leina Miyazaki, Maho Takayama, Hiroyuki Koike, Masaki Kimura, Masahirop Enomura, Yun-Wen Zheng, Keisuke Sekine, and Hideki Taniguchi

11. The Generation and Maintenance of Rat Induced Pluripotent Stem Cells

Tomoyuki Yamaguchi, Sanae Hamanaka, and Hiromitsu Nakauchi

12. Protocol for Cutaneous Wound Healing Assay in a Murine Model

Gitali Ganguli-Indra

13. Adipose Derived Stem Cells: Methods for Isolation and Applications for Clinical Use

Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok, Anne M. Wallace, and Steven Cohen

14. In vitro Detection of Residual Undifferentiated Cells in Retinal Pigment Epithelial Cells Derived from Human Induced Pluripotent Stem Cells

Takuya Kuroda, Satoshi Yasuda, and Yoji Sato

15. Whole Mount Immunohistochemistry to Study Spermatological Stem Cells and Spermatogenic Lineage in Mice, Monkeys and Humans

Kathrin Gassei, Hanna Valli, and Kyle E. Orwig

16. Differentiating the Stem Cell Pool of Human Hair Follicle Outer Root Sheath into Functional Melanocytes

Marie Schneider, Christina Dieckmann, Kathrin Rabe, Jan-Christoph Simon, and Vuk Savkovic

17. Pancreas Development ex vivo: Culturing Embryonic Pancreas Explants on Permeable Culture Inserts, with Firbonectin-Coated Glass Microwells, or Embedded in Three-Dimensional MatrigelTM

Hung Ping Shih and Maike Sander

18. Ultra-Rapid Manufacturing of Engineered Epicardial Substitute to Regenerate Cardiac Tissue Following Acute Ischemic Injury

Vahid Serpooshan and Pilar Ruiz-Lozano