The P2x7 Receptor: Methods and Protocols (Hardcover) (ISBN-13: 9781071623831)

1. How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating

Steven E. Mansoor

2. Agonists, Antagonists, and Modulators of P2X7 Receptors

Christa E. Müller and Vigneshwaran Namasivayam

3. Modulation of Cell Energy Metabolism by the P2X7 Receptor

Francesco Di Virgilio, Simonetta Falzoni, Alba Clara Sarti, Paola Chiozzi, Valentina Vultaggio-Poma, and Anna Lisa Giuliani

4. Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE

Sudad Dayl and Ralf Schmid

5. Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4)

Amal Elhage, Ross J. Turner, Peter Cuthbertson, Debbie Watson, and Ronald Sluyter

6. Development of Antibody and Nanobody Tools for P2X7

Tobias Stähler, Welbeck Danquah, Melanie Demeules, Henri Gondé, Romain Hardet, Friedrich Haag, Sahil Adriouch, Friedrich Koch-Nolte, and Stephan Menzel

7. Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors

Anna Marei Mann, Waldemar Schäfer, Sahil Adriouch, Kathleen Börner, Dirk Grimm, Ingke Braren, and Friedrich Koch-Nolte

8. Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations

9. Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes

Günther Schmalzing and Fritz Markwardt

10. A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors

Anna Durner and Annette Nicke

11. Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents

Xin Liang, Laura Janks, and Terrance M. Egan

12. Using Symmetrical Organic Cation Solutions to Study P2X7 Ion Permeation

Kate Dunning, Laurie Peverini, and Thomas Grutter

13. Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors

Ruth D. Murrell-Lagnado and Toshimitsu Kawate

14. Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry

Samantha Wittke, Leif Ancker, Sana Javed, and Friedrich Haag

15. Characterization of P2X7 Receptors in Human Blood Cells

Laura Hurtado-Navarro, Alberto Baroja-Mazo, and Pablo Pelegrín

16. Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in M

Author: Annette Nicke
ISBN-10: 1071623834
ISBN-13: 9781071623831
Publisher: Humana
Language: English
Published: 07/02/2022
Pages: 394
Format: Hardcover
Weight: 2.04lbs
Size: 10.00h x 7.00w x 0.94d