Viral Vectors for Gene Therapy: Methods and Protocols (Paperback) (ISBN-13: 9781493958283)

1. Introduction to Viral Vectors

James N. Warnock, Claire Daigre, and Mohamed Al-Rubeai

2. Introduction to Gene Therapy: A Clinical Aftermath

Patrice P. Denèfle

3. Host Cells and Cell Banking

Glyn N. Stacey and Otto-Wilhelm Merten

4. Overview of Current Scalable Methods for Purification of Viral Vectors

María Mercedes Segura, Amine Kamen, and Alain Garnier

5. Methods to Construct Recombinant Adenovirus Vectors

Miguel Chillon and Ramon Alemany

6. Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus

Edwige Dormond and Amine A. Kamen

7. Manufacturing of Retroviruses

Pedro E. Cruz, Teresa Rodrigues, Marlene Carmo, Dagmar Wirth, Ana Amaral, Paula M. Alves, and Ana S. Coroadinha

8. Lentiviral Vectors

Marc Giry-Laterrière, Els Verhoeyen, and Patrick Salmon

9. Adeno-Associated Viruses

Mauro Mezzina and Otto-Wilhelm Merten

10. Manufacturing of Adeno-Associated Viruses, for Example: AAV2

Haifeng Chen

11. Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses

J. Fraser Wright and Olga Zelenaia

12. Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells

Kari J. Airenne, Kaisa-Emilia Makkonen, Anssi J. Mähönen, and Seppo Ylä-Herttuala

13. Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors

Cornel Fraefel, Peggy Marconi, and Alberto L. Epstein

14. Manufacture of Measles Viruses

Kirsten K. Langfield, Henry J. Walker, Linda C. Gregory, and Mark J. Federspiel

15. In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model

Cecilia Frecha, Floriane Fusil, François-Loïc Cosset, and Els Verhoeyen

16. In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair)

Kolja Gelse and Holm Schneider

17. Ethical Consideration

Michael Fuchs

18. Clinical Trials of GMP Products in the Gene Therapy Field

Kathleen B. Bamford